SGER: Assessing Genetic Mechanisms of DNA Repair in Ancient Ice Microbes through Analytical Flow Cytometry, High-Speed Cell Sorting, and Single Cell Genomics

SGER:通过分析流式细胞术、高速细胞分选和单细胞基因组学评估古代冰微生物 DNA 修复的遗传机制

基本信息

  • 批准号:
    0907846
  • 负责人:
  • 金额:
    $ 19.46万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-01-01 至 2011-12-31
  • 项目状态:
    已结题

项目摘要

Technological advances in analytical flow cytometry and cell sorting are rapidly revolutionizing our understanding of microbial genetic diversity and its relationship to function in widely varied environmental systems. The in situ application of flow cytometry and high-speed sorting is now an indispensable component for interrogation of the physiological state and activity of subpopulations and assessment of the importance of specific genes to cell function. We propose to apply emerging techniques in analytical flow cytometry, high-speed cell sorting, and single-cell multiple displacement amplification (MDA) to Antarctic ice samples ranging 100 Kyr to 8 Myr in order to assess the relative proportion of viable vs. dead cells and to explore genetic strategies of DNA repair in ancient ice microbes. Recovery of viable microbes within ice exposed to cosmic radiation for ~8 Myr suggests that subpopulations of microbes within ancient ice possess particularly efficient DNA repair mechanisms. Given the strong influence of cosmic flux on DNA degradation in ancient ice samples and evidence of effective DNA repair, we hypothesize that the degree of DNA damage increases with time and that viable 8 Myr old bacteria possess particularly effective and novel DNA repair mechanisms for which we can obtain genetic signatures. The research goal is to obtain a library of genes used for DNA repair by viable bacteria from the oldest ice on earth. Using analytical techniques not previously applied to ancient ice microbes. Thus, the proposed research is an 'exploratory' endeavor with potentially high risk. It is likely that novel genetic capabilities will be discovered through our analyses, perhaps providing key molecular insight into the survival of life over geological time, and perhaps on other planets. Given the use of standard flow cytometry instrumentation and the increasing affordability of genome analyses, our research strategy is transferable to the scientific community and should offer key advantages to the study of ice microbes. This project will allow for continued professional development of a young PI and provide an opportunity whereby researchers with different educational backgrounds (undergraduate students, graduate students, technician, post-docs, and faculty) can interact and develop.
分析流式细胞术和细胞分选方面的技术进步正在迅速改变我们对微生物遗传多样性及其与各种环境系统中功能的关系的理解。流式细胞术和高速分选的原位应用是询问亚群的生理状态和活性以及评估特定基因对细胞功能的重要性的不可或缺的组成部分。我们建议将分析流式细胞术、高速细胞分选和单细胞多重置换扩增(MDA)中的新兴技术应用于100Kyr到8Myr的南极冰样,以评估活细胞与死亡细胞的相对比例,并探索古代冰微生物DNA修复的遗传策略。暴露在宇宙辐射中约8Myr的冰中活微生物的恢复表明,古冰中的微生物亚群具有特别有效的DNA修复机制。鉴于宇宙通量对古代冰样中DNA降解的强烈影响以及DNA有效修复的证据,我们假设DNA损伤的程度随着时间的推移而增加,活的8 Myr老细菌具有特别有效和新颖的DNA修复机制,我们可以获得其遗传特征。这项研究的目标是从地球上最古老的冰层中获得用于活细菌修复DNA的基因库。使用以前不适用于古代冰上微生物的分析技术。因此,拟议的研究是一项具有潜在高风险的“探索性”努力。通过我们的分析,很可能会发现新的遗传能力,也许会为生命在地质时期的生存提供关键的分子洞察力,也许还会在其他星球上提供。鉴于标准的流式细胞仪的使用和基因组分析的日益可负担性,我们的研究战略可以转移到科学界,并应该为冰微生物的研究提供关键优势。这个项目将允许年轻的PI继续专业发展,并提供一个机会,让不同教育背景的研究人员(本科生、研究生、技术员、博士后和教职员工)可以互动和发展。

项目成果

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Kay Bidle其他文献

Kay Bidle的其他文献

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{{ truncateString('Kay Bidle', 18)}}的其他基金

GCR: Collaborative Research: The Convergent Impact of Marine Viruses, Minerals, and Microscale Physics on Phytoplankton Carbon Sequestration
GCR:合作研究:海洋病毒、矿物质和微尺度物理对浮游植物碳固存的综合影响
  • 批准号:
    2021032
  • 财政年份:
    2020
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Continuing Grant
EDGE CT: Virus-inspired, lipid-mediated transfection and genetic manipulation of the marine coccolithophore, Emiliania huxleyi
EDGE CT:病毒启发、脂质介导的海洋颗石藻(Emiliania huxleyi)转染和基因操作
  • 批准号:
    1923297
  • 财政年份:
    2019
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Standard Grant
2018 Gordon Research Seminar and Conference on Marine Microbes: Italy - July 2018
2018 年戈登海洋微生物研究研讨会和会议:意大利 - 2018 年 7 月
  • 批准号:
    1839953
  • 财政年份:
    2018
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Standard Grant
Collaborative Research: Quantifying competing loss rates of viral lysis and microzooplankton grazing on Emiliania huxleyi mortality
合作研究:量化病毒裂解和微型浮游动物放牧对艾米利亚赫胥黎死亡率的竞争损失率
  • 批准号:
    1459200
  • 财政年份:
    2015
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Standard Grant
Collaborative Research: Elucidating algal host-virus dynamics in different nutrient regimes - mechanistic interactions and biogeochemical impact
合作研究:阐明不同营养状况下藻类宿主病毒的动态 - 机械相互作用和生物地球化学影响
  • 批准号:
    1537951
  • 财政年份:
    2015
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Standard Grant
Collaborative Research: Lipid lubrication of oceanic carbon and sulfur biogeochemistry via a host-virus chemical arms race
合作研究:通过宿主病毒化学军备竞赛进行海洋碳和硫生物地球化学的脂质润滑
  • 批准号:
    1061883
  • 财政年份:
    2011
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Standard Grant
Collaborative Research: A Matter of Life or Death? Assessing the physiological roles of PCD-related genes to stress adaptation in diatoms
合作研究:生死攸关?
  • 批准号:
    0927829
  • 财政年份:
    2009
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Standard Grant
The Role of Metacaspases in Mediating Cell Fate During Viral Infection of Unicelluar, Marine Phytoplankton
单细胞、海洋浮游植物病毒感染期间元半胱天冬酶在介导细胞命运中的作用
  • 批准号:
    0717494
  • 财政年份:
    2007
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Continuing Grant
An Examination of the Autocatalytic Cell Death Machinery in Marine, Planktonic Photoautotrophs
海洋浮游光合自养生物自催化细胞死亡机制的检查
  • 批准号:
    0414536
  • 财政年份:
    2004
  • 资助金额:
    $ 19.46万
  • 项目类别:
    Standard Grant

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