DISSERTATION RESEARCH: Incorporating Metagenomics into Experimental Community Ecology: Tests with the Pitcher Plant Model System

论文研究：将宏基因组学纳入实验群落生态学：用猪笼草模型系统进行测试

基本信息

批准号：
0909830
负责人：
Dianna Padilla
金额：
$ 1.5万
依托单位：
SUNY at Stony Brook
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0909830&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Incorporating Metagenomics into

项目摘要

Microbial systems, including the community of microbes that form the aquatic food web within the leaves of the pitcher plant, Sarracenia purpurea, have been important models for ecological studies. This is because they are easily replicated in the laboratory and have rapid dynamics that allow questions to be addressed in a matter of days that would take weeks to years with other types of organisms. To date, studies of the community ecology of microbial systems have been limited to those bacterial species that can be grown in the lab (culturable on agar), which is estimated to be less than 1% of the diversity within a community. The use of molecular biology techniques has revolutionized this field; microbial systems can now be studied by examining the genomes of all of the bacteria within these systems (metagenomics). This project will use metagenomics as a tool to identify bacterial species in a series of experiments designed to test questions about the dynamics of the food web of the pitcher plant system, and how this system responds to different types of impacts, including invasion by novel species. This project will also test whether the results of prior work (with only culturable bacteria) on the interactions and diversity of this system are robust when all bacteria in the system are considered. This project will train a female graduate student and undergraduates in molecular biology, community ecology and experimental design. This cross-disciplinary training will prepare them for careers multiple areas of the sciences.

微生物系统，包括在投手植物Sarracenia purpurea中形成水生食物网的微生物社区，是生态学研究的重要模型。这是因为它们很容易在实验室中复制，并且具有快速的动态，可以在几天之内解决问题，这可能需要数周到几年的其他类型的生物。迄今为止，对微生物系统的社区生态学的研究仅限于可以在实验室中种植的细菌物种（可在琼脂上培养），估计不到社区中多样性的1％。分子生物学技术的使用彻底改变了这一领域。现在可以通过检查这些系统中所有细菌的基因组（元基因组学）来研究微生物系统。该项目将使用宏基因组学作为一种工具来鉴定细菌物种在一系列实验中，旨在测试有关投手植物系统食品网络动力学的问题，以及该系统如何应对不同类型的影响，包括新物种的侵袭。当考虑系统中的所有细菌时，该项目还将测试该系统相互作用和多样性的先前工作结果（仅具有可培养细菌的结果）是否稳健。该项目将培训一名女性研究生，并在分子生物学，社区生态学和实验设计方面培训本科生。这种跨学科的培训将使他们为职业的多个科学领域做好准备。