Generating new biological component functions through RNA switches

通过RNA开关产生新的生物成分功能

• 批准号: 0917638
• 负责人: Christina Smolke
• 金额: $ 38.2万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0917638&HistoricalAwards=false
• 关键词: Generating; new; biological; component; functions

CBET-0828663Christina D Smolke, California Institute of TechnologyIntellectual merit RNA switches provide a genetically-encoded technology for programming information processing and control in living systems. This project advances a modular, extensible framework for the programming of biological control systems, in which target expression levels can be up- or down-regulated in response to molecular inputs and the regulatory response properties can be tuned for specific applications, thereby expanding the utility of the platform to a broader range of applications. By providing a general framework through which to link diverse molecular information to cellular phenotype, RNA switches provide powerful strategies for generating new biological components exhibiting a variety of sensory, enzymatic, or regulatory activities. This project emphasizes the advancement of an enabling technology that has well-defined design strategies and that is transferable to a variety of different systems, increasing the utility to and impact on the larger scientific community. Broader impacts Educational programs will be developed that merge research and education across different training levels, engage interdisciplinary teams of student researchers and mentors in open-ended research challenges and involve them in early phases of project definition and design, and emphasize community building and communicating research to different audiences. The programs will introduce cutting-edge engineering research to younger students and actively target involvement of underrepresented groups. The benefits of the research program to society are far-reaching as the resulting technologies will significantly impact our ability to interface with and engineer living systems.

CBET-0828663克里斯蒂娜D Smolke，加州理工学院智力价值RNA开关提供了一种遗传编码技术，用于编程信息处理和控制生命系统。该项目提出了一个模块化的，可扩展的框架，用于生物控制系统的编程，其中目标表达水平可以响应于分子输入而上调或下调，并且可以针对特定应用调整调节响应特性，从而将平台的实用性扩展到更广泛的应用范围。通过提供一个通用的框架，通过该框架将不同的分子信息与细胞表型联系起来，RNA开关提供了强大的策略，用于产生表现出各种感觉、酶或调节活性的新生物组分。该项目强调推进一项具有明确设计战略的使能技术，该技术可转移到各种不同的系统，增加对更大科学界的效用和影响。将制定教育计划，将不同培训级别的研究和教育合并，让学生研究人员和导师的跨学科团队参与开放式研究挑战，并让他们参与项目定义和设计的早期阶段，并强调社区建设和向不同受众传播研究。该计划将向年轻学生介绍尖端的工程研究，并积极针对代表性不足的群体的参与。研究计划对社会的好处是深远的，因为由此产生的技术将显著影响我们与生命系统的接口和工程设计的能力。