CAREER: Programming dynamic cellular response and logical processing with RNA sensors

这项研究的目标是为设计和建造量身定制的分子传感器开发平台，这些传感器将用于编程动态细胞行为。特别是，这些传感器将用于构建用于编程逻辑细胞响应的信号集成方案。此外，这项研究还包括一项综合教育和研究计划，该计划将为培养科学家和工程师建立新的战略，并为他们推动科学发展和技术向前发展奠定必要的基础。具体而言，本计划提出：(1)开发一种高通量策略，用于生成反式作用、配体响应的RNA开关；(2)开发设计顺式作用、配体响应的RNA开关的平台；(3)将这些传感器元件集成到逻辑处理方案的设计中，该方案根据接收到的输入信号组合对不同的细胞功能进行编程；(4)巩固生物分子和细胞工程的坚实基础，并通过尖端的综合研究和教育计划培训和教育科学家，鼓励其发展。

0545987SmolkeThe goal of this research is to develop platforms for the design and construction of tailor-made molecular sensors that will be used for programming dynamic cellular behavior. In particular, these sensors will be used to construct signal integration schemes for programming logical cellular response. In addition, this research includes an integrative educational and research plan that will build new strategies for educating scientists and engineers and lay the foundation necessary for them to push the boundaries of scientific development and technology forward. Specifically, this plan proposes to: (1) develop a high-throughput strategy for the generation of trans-acting, ligand-responsive RNA switches, (2) develop platforms for the design of cis-acting, ligand-responsive RNA switches, (3) integrate these sensor elements into the design of logical processing schemes that program different cellular functions depending on combinations of input signals received, and (4) solidify a strong base in biomolecular and cellular engineering and encourage its advancement by training and educating scientists through cutting-edge, integrated research and educational plans.