CAREER: Programming dynamic cellular response and logical processing with RNA sensors

这项研究的目标是为设计和建造定制的分子传感器开发平台，这些传感器将用于对动态细胞行为进行编程。特别是，这些传感器将被用来构建信号集成方案，以编程逻辑细胞响应。此外，这项研究包括一个综合的教育和研究计划，该计划将建立培养科学家和工程师的新战略，并为他们推动科学发展和技术进步奠定必要的基础。具体地说，该计划建议：(1)开发高通量策略以产生反式作用、配体响应型RNA开关，(2)开发顺式作用、配体响应型RNA开关的设计平台，(3)将这些传感器元件集成到逻辑处理方案的设计中，该逻辑处理方案根据接收到的输入信号的组合来编程不同的细胞功能，以及(4)巩固生物分子和细胞工程的坚实基础，并通过尖端、集成的研究和教育计划培训和教育科学家，鼓励其进步。

0545987SmolkeThe goal of this research is to develop platforms for the design and construction of tailor-made molecular sensors that will be used for programming dynamic cellular behavior. In particular, these sensors will be used to construct signal integration schemes for programming logical cellular response. In addition, this research includes an integrative educational and research plan that will build new strategies for educating scientists and engineers and lay the foundation necessary for them to push the boundaries of scientific development and technology forward. Specifically, this plan proposes to: (1) develop a high-throughput strategy for the generation of trans-acting, ligand-responsive RNA switches, (2) develop platforms for the design of cis-acting, ligand-responsive RNA switches, (3) integrate these sensor elements into the design of logical processing schemes that program different cellular functions depending on combinations of input signals received, and (4) solidify a strong base in biomolecular and cellular engineering and encourage its advancement by training and educating scientists through cutting-edge, integrated research and educational plans.