Evolution of persistence in the model bacterium, Sinorhizobium

模型细菌中华根瘤菌的持久性进化

• 批准号: 0918897
• 负责人: Robert Ford Denison
• 金额: $ 29.89万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0918897&HistoricalAwards=false
• 关键词: Evolution; persistence; model; bacterium; Sinorhizobium

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Some bacteria can enter a nongrowing "persister" state that allows them to survive antibiotics and other treatments that normally kill them. By suspending growth, they may also free resources for their genetically identical clonemates.Most species form only a few persisters. This makes persisters hard to study, despite their importance in long-term infections. However, certain harmless bacteria from plant roots can form up to 40% persisters. These will be used to determine whether persisters benefit mainly from enhanced stress resistance or by increasing the growth of their clonemates.Successful completion of this research will provide two main benefits: First, this research will determine the conditions that favor the spread of persister-forming bacterial strains over nonpersister strains, and the genetic basis of persistence. This can provide direct medical benefits by aiding the development of novel management strategies, drug targets, and eventually treatments for patients infected with persister-forming bacteria. Second, some conclusions may apply to other species that are difficult to eradicate because they, too, form dormant, stress-resistant stages. These include many agricultural weeds and some species of mosquito. One key advantage of the proposed approach is speed: experiments that would take decades with weeds or mosquitoes can be conducted in months with bacteria. This research will provide training opportunities and jobs for undergraduates, high school students, and a post doctoral researcher.

该奖项是根据2009年的《美国复苏与再投资法》（公法111-5）资助的。一种细菌可以进入一个不添加“持久”状态，使他们能够在通常杀死他们的抗生素和其他治疗方法中生存。通过暂停增长，它们也可以为其遗传相同的克隆人提供免费资源。大多数物种仅形成了少数言论。 尽管在长期感染中重要性，但这使坚持不懈地研究。 但是，植物根部的某些无害细菌最多可能形成40％的持久性。 这些将用于确定毅力是否主要从增强的压力抗性中受益或增加其克隆人的生长。完成这项研究的成功完成将带来两个主要好处：首先，这项研究将确定有利于持久形成的细菌菌株超过非百叶酱菌株的传播的条件，以及持久性的遗传基础。 这可以通过帮助开发新的管理策略，药物目标以及最终对感染持久形成细菌的患者的治疗来提供直接的医疗益处。 其次，一些结论可能适用于难以消除的其他物种，因为它们也形成了休眠，耐心的阶段。 这些包括许多农业杂草和一些蚊子。拟议方法的一个主要优点是速度：可以用细菌在几个月内使用杂草或蚊子进行数十年的实验。这项研究将为大学生，高中生和博士后研究员提供培训机会和工作。