Evolution of persistence in the model bacterium, Sinorhizobium

模型细菌中华根瘤菌的持久性进化

• 批准号: 0918897
• 负责人: Robert Ford Denison
• 金额: $ 29.89万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0918897&HistoricalAwards=false
• 关键词: Evolution; persistence; model; bacterium; Sinorhizobium

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Some bacteria can enter a nongrowing "persister" state that allows them to survive antibiotics and other treatments that normally kill them. By suspending growth, they may also free resources for their genetically identical clonemates.Most species form only a few persisters. This makes persisters hard to study, despite their importance in long-term infections. However, certain harmless bacteria from plant roots can form up to 40% persisters. These will be used to determine whether persisters benefit mainly from enhanced stress resistance or by increasing the growth of their clonemates.Successful completion of this research will provide two main benefits: First, this research will determine the conditions that favor the spread of persister-forming bacterial strains over nonpersister strains, and the genetic basis of persistence. This can provide direct medical benefits by aiding the development of novel management strategies, drug targets, and eventually treatments for patients infected with persister-forming bacteria. Second, some conclusions may apply to other species that are difficult to eradicate because they, too, form dormant, stress-resistant stages. These include many agricultural weeds and some species of mosquito. One key advantage of the proposed approach is speed: experiments that would take decades with weeds or mosquitoes can be conducted in months with bacteria. This research will provide training opportunities and jobs for undergraduates, high school students, and a post doctoral researcher.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。一些细菌可以进入一种非生长的“持久”状态，使它们能够在抗生素和其他通常杀死它们的治疗中存活。通过停止生长，它们也可以为基因相同的克隆体释放资源。 这使得坚持者很难研究，尽管他们在长期感染中很重要。 然而，某些来自植物根部的无害细菌可以形成高达40%的坚持者。 这些将被用来确定是否persisters主要受益于增强的抗逆性或通过增加其clonemates的增长。成功完成这项研究将提供两个主要好处：首先，这项研究将确定有利于持久形成细菌菌株传播的条件，而不是nonpersister菌株，以及持久性的遗传基础。 这可以通过帮助开发新的管理策略、药物靶点以及最终治疗感染持久性形成细菌的患者来提供直接的医疗益处。 第二，有些结论可能适用于其他难以根除的物种，因为它们也形成了休眠的抗应激阶段。 这些包括许多农业杂草和一些种类的蚊子。该方法的一个关键优势是速度：用杂草或蚊子进行数十年的实验可以在几个月内用细菌进行。本研究将为本科生、高中生和博士后研究人员提供培训机会和就业机会。