RUI: Investigation of the mechanisms that regulate interactions between the transcription elongation factor Spt16 and chromatin.

RUI：研究调节转录延伸因子 Spt16 和染色质之间相互作用的机制。

• 批准号: 0919241
• 负责人: Andrea Duina
• 金额: $ 47.31万
• 依托单位: Hendrix College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2013-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0919241&HistoricalAwards=false
• 关键词: RUI; Investigation; mechanisms; regulate; interactions

The genetic information carried by all living cells is contained within long molecules of deoxyribonucleic acid (DNA). In eukaryotic cells, most of the DNA is localized within a cellular compartment referred to as the cell nucleus. In order to fit within the small confines of the nucleus, DNA associates with a number of proteins to form a highly compacted structure known as chromatin. Whereas this compaction is essential for life, it also represents a physical barrier to those factors that bind DNA and that are required for the initial steps in the conversion of genetic information into specific biological events, a process more generally known as gene transcription. In recent years it has become clear that cells utilize a number of chromatin-altering proteins that can interact with and change the structure of chromatin to make the DNA more or less accessible to factors needed for transcription. In this project a combination of genetic and biochemical strategies will be used to elucidate the mechanisms that regulate how one of these chromatin-altering proteins, known as Spt16, interacts with chromatin during the process of gene transcription. Because accurate regulation of transcription is crucial for the proper function of all cells and organisms, these studies promise to provide new insights into a basic biological process of general importance.Broader impacts. This project will provide an excellent opportunity for undergraduate students to be actively involved in cutting-edge genetic and biochemical research. During this project, undergraduate students will be trained on important aspects of scientific research, including the proper design and execution of experiments, accurate analysis and interpretation of experimental data, and the development of scientific writing and oral communication skills. In addition, undergraduate students involved in this project will have the opportunity to present the results of their research at regional, national and international scientific meetings. These combined experiences will not only introduce undergraduate students to the exciting world of scientific research, but will also better prepare them for making informed decisions regarding their career paths following graduation from college.

所有活细胞所携带的遗传信息都包含在脱氧核糖核酸（DNA）的长分子中。 在真核细胞中，大部分DNA位于称为细胞核的细胞区室中。 为了适应细胞核的小范围，DNA与许多蛋白质结合，形成高度致密的结构，称为染色质。 虽然这种压缩对生命至关重要，但它也代表了对那些结合DNA的因子的物理屏障，这些因子是将遗传信息转化为特定生物事件的初始步骤所必需的，这一过程通常称为基因转录。 近年来，已经清楚的是，细胞利用许多染色质改变蛋白，这些蛋白可以与染色质相互作用并改变染色质的结构，使DNA或多或少地接近转录所需的因子。在这个项目中，遗传和生化策略的组合将被用来阐明调节这些染色质改变蛋白之一，称为Spt16，在基因转录过程中与染色质相互作用的机制。 由于转录的精确调控对于所有细胞和生物体的正常功能至关重要，这些研究有望为具有普遍重要性的基本生物学过程提供新的见解。 该项目将为本科生提供一个积极参与尖端遗传和生物化学研究的绝佳机会。 在这个项目中，本科生将接受科学研究的重要方面的培训，包括正确的实验设计和执行，准确的分析和解释实验数据，以及科学写作和口头交流技能的发展。 此外，参与该项目的本科生将有机会在区域，国家和国际科学会议上展示他们的研究成果。 这些综合经验不仅将本科生介绍给科学研究的令人兴奋的世界，但也将更好地准备他们从大学毕业后，就自己的职业道路作出明智的决定。