Integrated Calcium and pH Signaling in C. elegans

秀丽隐杆线虫中的整合钙和 pH 信号传导

• 批准号: 0919848
• 负责人: Keith Nehrke
• 金额: $ 56.49万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0919848&HistoricalAwards=false
• 关键词: Integrated; Calcium; pH; Signaling; C.

Keith NehrkeProposal Number: IOS-0919848Award title: Integrated Calcium and pH Signaling in C. elegansA major challenge in physiology is bridging the gap between molecular and integrative functions, or how processes occurring at the cell level are organized to convey systems functions. The genetic model organism C. elegans exhibits a rhythmic behavior that occurs every 45 seconds, and a variety of cell signaling processes contribute to the behavioral output. The project is designed to exploit the power of the nematode model system to explore functional interactions between these processes. The project will also study a novel mechanism for fast cell-cell communication, which forms the basis for one component of the rhythmic behavior. The investigators will employ during the course of these studies fluorescent biosensors to measure cell signaling in live worms, as well as pharmacology, genetics, and molecular techniques to address how cell signaling at the molecular level is translated into behavior in live organisms. This work could potentially transform our view of cell signaling, leading to the emergence of a new area of study with important implications for how cells communicate with one another to convey behavior. The project will provide training for two Ph.D. students and summer research opportunities for several undergraduate students, with a strong emphasis on minority applicants. As a part of this project, the investigators will also create and maintain a website that can be accessed and understood by the layperson.

基思·尼克尔提案编号：IOS-0919848奖项名称：C.生理学的一个主要挑战是弥合分子和整合功能之间的差距，或者细胞水平上发生的过程如何组织以传达系统功能。遗传模式生物C.秀丽隐杆线虫每45秒发生一次有节奏的行为，各种细胞信号传导过程促成了行为输出。该项目旨在利用线虫模型系统的力量来探索这些过程之间的功能相互作用。该项目还将研究一种快速细胞间通讯的新机制，这是节奏行为的一个组成部分的基础。研究人员将在这些研究过程中使用荧光生物传感器来测量活蠕虫中的细胞信号，以及药理学，遗传学和分子技术来解决分子水平上的细胞信号如何转化为活生物体中的行为。这项工作可能会改变我们对细胞信号传导的看法，导致一个新的研究领域的出现，对细胞如何相互交流以传达行为具有重要意义。该项目将为两名博士提供培训。学生和暑期研究机会，为几个本科生，重点是少数民族申请人。作为该项目的一部分，调查人员还将创建和维护一个可供外行访问和理解的网站。