Trial manufacture of a miniaturized calcium-ion electrode, and its practical application to determine critical pH and to develop a new evaluation method of food cariogenicity

微型钙离子电极的试制及其临界pH测定及食品致龋性评价新方法的实际应用

• 批准号: 10557168
• 负责人: YAMADA Tadashi
• 金额: $ 7.36万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10557168/
• 关键词: miniaturized calcium-ion electrode; ion-sensitive field-effect transistor electrode; calcium-ion concentration; critical pH; cariogenicity; hydrogen-ion concentration; evaluation method of cariogenicity

By covering the PVC membrane including calcium-ionophore to the ion-sensitive field-effect transistor (ISFET) electrode directly, we made the calcium ISFET electrode. Though we tried to change the thickness of PVC membrane and the kind of ionophore, we were unable to make the calcium-ion electrode that is not influenced by pH.Therefore, the silicon nitride membrane (the hydrogen-ion reaction membrane) was removed from the surface of the ISFET electrode, and Parylene C was deposited instead of the silicon nitride, and then the PVC membrane was covered on it. When the calcium ion of various concentrations were measured by using the improved electrode the potential response of the straight line was observed the range of 2 mmol/L from 1μmol/L of calcium ion. Next, we examined the influence of pH to calcium ion measurement. As calcium-ion concentration becomes high, pH hardly affected the potential response to calcium ion. And it became clear to hardly receive the influence of pH in 1μmol/L as calcium-ion concentration. It is considered that calcium ion is able to measured without receiving the influence of pH in the dental plaque where calcium ion concentration could be higher than 1μmol/L.When calcium ion was measured with the electrode of silicon nitride membrane covering, the hindrance of the sodium ion was hardly observed (less than 40 mmol/L). However, it was hindered by the sodium ion of low concentration (less than 10 mmol/L), when we used the electrode of Parylene C covering. The manufacture of the practical electrode that excels in the potential response to calcium ion without receiving the influence of sodium ion, is necessary, to measure the calcium ion in dental plaque precisely. It is because sodium-ion concentration is about 8 mmol/L in unstimulated saliva and about 30 mmol/L in stimulated saliva.

通过将包含钙离子载体的PVC膜直接覆盖到离子敏感场效应晶体管（ISFET）电极上，我们制成了钙ISFET电极。尽管我们尝试改变PVC膜的厚度和离子载体的种类，但无法制作出不受pH影响的钙离子电极。因此，从ISFET电极表面去除氮化硅膜（氢离子反应膜），并沉积Parrene C代替氮化硅，然后在其上覆盖PVC膜。使用改进的电极测量不同浓度的钙离子时，在钙离子1μmol/L至2mmol/L的范围内观察到直线电位响应。接下来，我们研究了 pH 对钙离子测量的影响。随着钙离子浓度变高，pH值几乎不影响钙离子的潜在反应。可知钙离子浓度在1μmol/L时几乎不受pH的影响。认为钙离子的测量不受牙菌斑pH值的影响，牙菌斑中的钙离子浓度可高于1μmol/L。当用氮化硅膜覆盖的电极测量钙离子时，几乎观察不到钠离子的阻碍（小于40mmol/L）。然而，当我们使用Parylene C覆盖的电极时，它会受到低浓度（小于10 mmol/L）钠离子的阻碍。为了精确测量牙菌斑中的钙离子，需要制造对钙离子具有优异的电位响应而不受钠离子影响的实用电极。这是因为未受刺激的唾液中的钠离子浓度约为8mmol/L，而受刺激的唾液中的钠离子浓度约为30mmol/L。

项目成果

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