The Involvement of Thiol-metabolizing Pathways in Thylakoid Biogenesis

硫醇代谢途径参与类囊体生物发生

• 批准号: 0920062
• 负责人: Patrice Hamel
• 金额: --
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920062&HistoricalAwards=false
• 关键词: Involvement; Thiol; metabolizing; Pathways; Thylakoid

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-15).Intellectual merit The long-term goal of this activity is to define novel, critical components required for the assembly of photosynthetic membranes. Photosynthesis is the major mechanism of solar energy capture in the biosphere, providing organic carbon for food, fuel and fiber. This research will pursue an important series of experiments to address the role of two proteins potentially required for thiol-redox chemistry in the thylakoid lumen. As thylakoids are essential to photosynthesis, defining how they are assembled and maintained, represents research fundamental to energy generation in the biosphere. This research is an emerging frontier in the biology of photosynthesis, and could also have impact on chloroplast metabolic engineering in agriculture. The proposed research will investigate two proteins that have been identified as potentially involved in thiol redox chemistry in the thylakoid lumen, using Chlamydomonas as a study organism. CCS5 is a lumen-facing thioredoxin-like protein implicated in assembly of the heme proteins cytochrome f and c6. The first aim will examine the redox potentials of CCS5 and the target proteins, test CCS5 for disulfide reductase activity, and determine the interaction of CCS5 and targets in vivo. The second aim will investigate LTO1, a candidate thiol-oxidizing protein. Biochemical and molecular genetic experiments will determine if the protein has the hypothesized activity. The emergence of thiol-reducing and thiol-oxidizing components for the manufacture of the thylakoid compartment is a recent and novel development in the field of membrane biogenesis.Broader impact:A central aspect of this project is its impact on undergraduate and graduate education, as well as curriculum enrichment. The proposed research employs advanced molecular genetic and biochemical approaches that will serve as the basis for training students in the fundamental area of organelle biology. In outreach efforts to offer summer research experiences to undergraduates with limited research opportunities, the PI has established a partnership with Transylvania University in rural Kentucky. In addition, the PI is developing a unique plan for educational outreach beyond institutional boundaries. This plan will target migrant high school students in Ohio, a Hispanic minority, academically neglected due to a mobile lifestyle. The PI will engage migrant students and teachers from a rural high school in various activities with the objective to encourage the target population to pursue post-secondary education in the sciences.

该奖项是根据2009年美国复苏和再投资法案（公法111-15）资助的。这项活动的长期目标是定义组装光合膜所需的新颖的关键成分。光合作用是生物圈捕获太阳能的主要机制，为食物、燃料和纤维提供有机碳。本研究将进行一系列重要的实验，以解决类囊体腔中硫醇氧化还原化学可能需要的两种蛋白质的作用。由于类囊体对光合作用至关重要，定义它们是如何组装和维持的，代表了生物圈中能量产生的基础研究。该研究是光合作用生物学的一个新兴前沿，也可能对农业叶绿体代谢工程产生影响。拟议的研究将以衣藻为研究对象，研究两种已被确定可能参与类囊体腔中硫醇氧化还原化学的蛋白质。CCS5是一种面向管腔的硫氧还蛋白样蛋白，参与血红素蛋白细胞色素f和c6的组装。第一个目的是检测CCS5和靶蛋白的氧化还原电位，测试CCS5的二硫还原酶活性，并确定CCS5与靶蛋白在体内的相互作用。第二个目的是研究候选的硫醇氧化蛋白LTO1。生化和分子遗传学实验将确定蛋白质是否具有假设的活性。巯基还原和巯基氧化组分的出现是膜生物发生领域的新进展。更广泛的影响：这个项目的一个中心方面是它对本科和研究生教育的影响，以及课程的丰富。建议的研究采用先进的分子遗传学和生物化学方法，将作为培养学生细胞器生物学基础领域的基础。为了向研究机会有限的本科生提供暑期研究经验，PI与肯塔基州农村的特兰西瓦尼亚大学建立了合作关系。此外，PI正在制定一项超越机构界限的独特教育推广计划。该计划将针对俄亥俄州的移民高中生，他们是西班牙裔少数民族，由于流动的生活方式而在学业上被忽视。该计划将让来自农村高中的移民学生和教师参与各种活动，目的是鼓励目标人口接受中学后的科学教育。