Epigenetic Landscapes and Transcriptomes in Two Maize Inbred Lines and Their Heterotic Hybrids

两个玉米自交系及其杂种的表观遗传景观和转录组

基本信息

  • 批准号:
    0922604
  • 负责人:
  • 金额:
    $ 50万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-09-15 至 2013-08-31
  • 项目状态:
    已结题

项目摘要

Heterosis (or hybrid vigor) is the phenomenon where the progeny of diverse inbred parents within a species have superior biomass, stature and/or fertility compared to either of the inbred parental lines. Although heterosis is commonly exploited in crop production, the molecular basis is largely unknown. This proposal will address whether small RNAs and epigenetic modifications (such as histone modifications and DNA methylation) are involved in regulation of heterosis in maize. This project will employ next-generation sequencing to provide genome-wide profiling of messenger RNA and small RNA transcriptomes and selected epigenetic modifications in two inbred lines and their reciprocal hybrid offspring. A systematic analysis of these data will provide genome-wide maps of DNA methylation, histone modification patterns, sequence polymorphisms, small RNA abundance, and gene expression levels in the maize inbred lines and their reciprocal hybrids. This in-depth analysis of a selected model pair of maize lines will aid in achieving the long-term goal of understanding the molecular mechanisms of heterosis in maize. Understanding the molecular events underlying heterosis has the potential to improve crop and livestock breeding programs. In addition, because heterosis is potentially a driving evolutionary force, elucidating its molecular basis will contribute to our understanding of the evolution of species. This project will provide training to young researchers at the postdoctoral, graduate, and undergraduate levels. In addition, the project will support an outreach program that couples undergraduate summer research projects with undergraduate classes and projects during the academic year. This project will be integrated into undergraduate bioinformatics and molecular biology curricula through class assignments and project-based lessons. Project personnel will also participate in various outreach events targeting younger students. A website (http://plantgenomics.biology.yale.edu) will be developed to provide public access to the data and provide a summary of the project. All datasets will be deposited at the NIH GEO repository (http://www.ncbi.nlm.nih.gov/geo) and the Plant Gene Expression database (PLEXdb) at http://plexdb.org.
杂种优势(或杂种优势)是指物种内不同自交系的后代与自交系中的任何一个相比具有更好的生物量、身高和/或育性的现象。虽然杂种优势在作物生产中被普遍利用,但其分子基础在很大程度上是未知的。这项建议将讨论小RNA和表观遗传修饰(如组蛋白修饰和DNA甲基化)是否参与玉米杂种优势的调节。该项目将采用下一代测序技术,在两个自交系及其相互杂交后代中提供信使RNA和小RNA转录本的全基因组图谱以及选定的表观遗传修饰。对这些数据的系统分析将提供玉米自交系及其正反交后代的DNA甲基化、组蛋白修饰模式、序列多态、小RNA丰度和基因表达水平的全基因组图谱。对选定的模式玉米品系的深入分析将有助于实现了解玉米杂种优势的分子机制这一长期目标。了解杂种优势背后的分子事件有可能改善作物和家畜育种计划。此外,由于杂种优势是潜在的进化驱动力,阐明其分子基础将有助于我们理解物种的进化。该项目将为年轻的博士后、研究生和本科生提供培训。此外,该项目还将支持一项外展计划,将本科生暑期研究项目与本学年的本科生课程和项目结合起来。这个项目将通过课堂作业和基于项目的课程整合到本科生物信息学和分子生物学课程中。项目人员还将参加针对年轻学生的各种外联活动。将开发一个网站(http://plantgenomics.biology.yale.edu)),供公众查阅数据并提供项目摘要。所有数据集将保存在美国国立卫生研究院地理信息库(http://www.ncbi.nlm.nih.gov/geo))和植物基因表达数据库(PLEXDB)中,网址为http://plexdb.org.

项目成果

期刊论文数量(0)
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专利数量(0)

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Xing-Wang Deng其他文献

Xing-Wang Deng的其他文献

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{{ truncateString('Xing-Wang Deng', 18)}}的其他基金

Virtual Center for Analysis of Rice Genome Transcription
水稻基因组转录分析虚拟中心
  • 批准号:
    0421675
  • 财政年份:
    2004
  • 资助金额:
    $ 50万
  • 项目类别:
    Continuing Grant
Molecular Genetic Analysis of Arabidopsis COP9 Signalosome
拟南芥COP9信号体的分子遗传学分析
  • 批准号:
    0077217
  • 财政年份:
    2000
  • 资助金额:
    $ 50万
  • 项目类别:
    Continuing Grant
Molecular and Genetic Analysis of the COP9 Complex, A Novel Light-Regulated Developmental Regulator in Arabidopsis
拟南芥中新型光调控发育调节因子 COP9 复合物的分子和遗传分析
  • 批准号:
    9513366
  • 财政年份:
    1996
  • 资助金额:
    $ 50万
  • 项目类别:
    Continuing Grant
Presidential Faculty Fellows
总统教职研究员
  • 批准号:
    9553030
  • 财政年份:
    1996
  • 资助金额:
    $ 50万
  • 项目类别:
    Continuing Grant
Molecular and Genetic Analysis of COP9, A Regulatory Component Involved in Light-Controlled Development in Arabidopsis
拟南芥光控发育调控元件 COP9 的分子和遗传分析
  • 批准号:
    9303029
  • 财政年份:
    1993
  • 资助金额:
    $ 50万
  • 项目类别:
    Continuing Grant

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