MRI: Acquisition of Instrumentation for a Molecular Ecology and Biosystematics Laboratory

MRI：为分子生态学和生物系统学实验室购置仪器

• 批准号: 0922603
• 负责人: Brian Teasdale
• 金额: $ 29.17万
• 依托单位: Kean University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922603&HistoricalAwards=false
• 关键词: MRI; Acquisition; Instrumentation; Molecular; Ecology

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Kean University has been awarded funds from the NSF to acquire equipment that will establish a core, multi-user DNA sequencing facility called Molecular Ecology and Biosystematics Laboratory (MEBL). The instrumentation includes: (1) an Applied Biosystems, four-capillary AB3130 Genetic Analyzer, with Sequencher 4.2 software workstation; (2) BioRad DNA Engine Peltier Thermal Cycler + Chromo-4 Real-Time PCR Detection System; (3) Eppendorf Refrigerated Microcentrifuge (Model 5417R); (4) Eppendorf Mastercyler Thermal Gradient Cycler (Model E5331000 045); (5) Fisher Scientific Isotemp Ultralow-Temperature Freezer (Model 13-990-32); (6) Perkin Elmer LAMBDA 950 Spectrophotometer w/ UV/VIS/NIR capabilities; and (7) Percival Scientific P-99 Walk-in Plant Growth Chamber with Advanced Intellius Controller and H-12 Relative Humidity Control Option. The equipment will be used by faculty and undergraduate students in research centered on the use of DNA sequence information. The research will address a broad range of questions related to the molecular ecology and biosystematics of: (1) plant-associated microfungi, (2) marine macroalgae, (3) melanthiaceous angiosperms and (4) hymenopteran insects in urban ecosystems. The instruments will be used for undergraduate student training, especially gender and ethnically underrepresented individuals who are interested in a career in the sciences. Kean University is ideally suited for this effort because of its focus on undergraduate education and its highly diverse and non-traditional student body, and because of its proximity to leading pharmaceutical and biotech industries in northern New Jersey. The MEBL will serve as a catalyst for the recruitment and subsequent employment of underrepresented student groups into the life sciences, in addition to forging cross-disciplinary ties between Biology, Chemistry and Computer Science Departments within the University. Additionally, the MEBL will serve as a foundation for the strengthening of pre-existing curricula, as well as the development of a set of new curricula specifically focused on the acquisition and analysis of DNA sequence information. Results from studies conducted with the new molecular ecology equipment will be published in peer-reviewed journals and disseminated through student and faculty presentations at regional or national meetings.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。基恩大学已获得NSF的资金，用于购买将建立一个核心的多用户DNA测序设施，称为分子生态学和生物系统学实验室（MEBL）。仪器包括：（1）Applied Biosystems，四毛细管AB 3130遗传分析仪，配备Sequencher 4.2软件工作站;（2）BioRad DNA Engine Peltier热循环仪+染色体-4实时PCR检测系统;（3）Eppendorf冷冻微量离心机（型号5417 R）;（4）Eppendorf Mastercyler热梯度循环仪（型号E5331000 045）;（5）Fisher Scientific Isotemp超低温冷冻箱（型号13-990-32）;（6）Perkin Elmer LAMBDA 950分光光度计w/ UV/维斯/NIR能力;和（7）Percival Scientific P-99步入式植物生长室，具有高级智能控制器和H-12相对湿度控制选项。该设备将被教师和本科生用于以DNA序列信息使用为中心的研究。该研究将解决与分子生态学和生物系统学有关的广泛问题：（1）植物相关的微型真菌，（2）海洋大型藻类，（3）黑色素被子植物和（4）城市生态系统中的hyperteran昆虫。这些工具将用于本科生培训，特别是对科学职业感兴趣的性别和种族代表性不足的个人。基恩大学非常适合这一努力，因为它专注于本科教育和高度多样化和非传统的学生群体，并因为它靠近北方新泽西领先的制药和生物技术行业。MEBL将作为招聘和随后的就业人数不足的学生群体进入生命科学的催化剂，除了锻造生物，化学和计算机科学部门之间的跨学科联系在大学。此外，MEBL将作为加强现有课程的基础，以及开发一套专门针对DNA序列信息获取和分析的新课程。 用新的分子生态学设备进行的研究结果将发表在同行评议的期刊上，并通过学生和教师在区域或国家会议上的演讲传播。