Functional Genomics of Maize Centromeres

玉米着丝粒的功能基因组学

• 批准号: 0922703
• 负责人: R Kelly Dawe
• 金额: $ 398.93万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-15 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922703&HistoricalAwards=false
• 关键词: Functional; Genomics; Maize; Centromeres

PI: R. Kelly Dawe (University of Georgia - Athens)CoPIs: James A. Birchler (University of Missouri - Columbia), Jiming Jiang (University of Wisconsin - Madison), Gernot Presting (University of Hawaii - Manoa), and Jeffrey Ross-Ibarra (University of California - Davis)Centromeres are regions of the genome that organize and regulate chromosome movement. They are key elements in the development of next-generation artificial chromosomes for introducing new traits into crop plants. Yet the biology of centromeres remains poorly understood. The experiments carried out in this project will complete the sequence and assembly of maize centromeres, address known epigenetic limitations associated with employing artificial chromosomes, develop improved strategies for streamlining centromere study and use, and document the diversity of centromeres in maize and allied species. To expand training opportunities in plant genetics, two programs will be initiated. One will provide hands-on training in plant cytogenetics through an Annual Cytogenetics Workshop to be held at the University of Wisconsin. The second will be an international student exchange program between project laboratories and the National Autonomous University of Mexico. Specifically, thirteen students will be funded to travel between universities and work on collaborative projects relating to centromere evolution and function. A web site (www.plantcentromeres.org) will be used to disseminate results from the project and to coordinate outreach activities. In addition, sequence data will be deposited at MaizeGDB (www.maizegdb.org) and GenBank. Germplasm generated will be deposited at the Iowa North Central Regional Plant Introduction Station (Ames, IA).

PI: R. Kelly Dawe（佐治亚大学雅典分校），CoPIs: James A. Birchler（密苏里大学哥伦比亚分校），jimming Jiang（威斯康星大学麦迪逊分校），Gernot Presting（夏威夷大学马诺阿分校），Jeffrey Ross-Ibarra（加州大学戴维斯分校）着丝粒是基因组中组织和调节染色体运动的区域。它们是开发下一代人工染色体以向作物引入新性状的关键因素。然而，对着丝粒的生物学仍然知之甚少。本项目所进行的实验将完成玉米着丝粒的序列和组装，解决与使用人工染色体相关的已知表观遗传限制，制定改进的策略来简化着丝粒的研究和使用，并记录玉米和相关物种中着丝粒的多样性。为了扩大植物遗传学方面的培训机会，将启动两个项目。一个将通过在威斯康星大学举行的年度细胞遗传学研讨会提供植物细胞遗传学的实践培训。第二个将是项目实验室与墨西哥国立自治大学之间的国际学生交换计划。具体来说，13名学生将获得资助，在各所大学之间旅行，从事与着丝粒进化和功能有关的合作项目。将利用一个网站（www.plantcentromeres.org）传播该项目的成果和协调外联活动。此外，序列数据将存入MaizeGDB （www.maizegdb.org）和GenBank。产生的种质将存放在爱荷华州北部中部地区植物引种站（Ames， IA）。