TRPGR: Maize Cell Genomics: Resources for Visualizing Promoter Activity and Protein Dynamics using Fluorescent Protein Lines

TRPGR：玉米细胞基因组学：使用荧光蛋白系可视化启动子活性和蛋白质动力学的资源

• 批准号: 1027445
• 负责人: David Jackson
• 金额: $ 538.88万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-10-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1027445&HistoricalAwards=false
• 关键词: TRPGR; Maize; Cell; Genomics; Resources

PI: David Jackson (Cold Spring Harbor Laboratory)Co-PIs: Anne Sylvester (University of Wyoming)Agnes Chan (The J. Craig Venter Institute)Intellectual Merit. Maize is a powerful model system for functional genomics in the grain crops, because its reference genome has recently been sequenced and functional tools are increasing. However, new methods of investigating gene function within cells and tissues are needed to understand protein functions at the cellular and sub-cellular level, which will ultimately provide guiding information for crop improvement. This project aims to generate tools in maize that can be used to drive expression of any gene in a number of specific chosen tissue or cell types. The use of these tools will permit the visualization of reporter genes with unprecedented spatial and temporal resolution, and will also enable functional gene over-expression or gene knockout studies. In addition, this project will make use of newly improved fluorescent protein color tags to label sub-cellular or developmental compartments, and will develop live imaging techniques for growing maize plants to demonstrate the utility of all of the reporter lines for functional studies. The project will therefore transform functional genetic experiments in maize, by providing a means to understand gene function in specific cells and tissues during development. The project will deliver to the research community a permanent stock of stably transformed seeds for 50 tissue specific activator/reporter lines, 20 fluorescent-tagged protein lines, as well as fully characterized in vivo imaging methods. It will also develop a pipeline for handling large image datasets that will be applicable to other projects. All resources, including images, seeds and gene constructs, will be publicly available via the project website http://maize.jcvi.org/cellgenomics. For long-term availability, images will be migrated to the community database, MaizeGDB, and seeds will be deposited with the Maize Genetics Stock Center. Broader Impacts. The project will integrate education and outreach by organizing an annual Genetics Workshop for Native American students at Little Big Horn College, a Tribal College to the Crow Nation. The project will also integrate functional research into educational tools by generating 3D interactive animations of a maize cell and growing plant, displaying sub-cellular compartments and gene expression data. The animations will be beta-tested by three target groups, including Native American students at Little Big Horn College, deaf and hard of hearing students in a cell biology class at Gallaudet University, and University of Wyoming biology students. Animations will be made publicly available and incorporated in course curricula taught at the participating institutions.

主要研究者：大卫杰克逊（冷泉港实验室）合作PI：安妮西尔维斯特（怀俄明州大学）艾格尼丝陈（J.克雷格文特尔研究所）智力功绩。 玉米是粮食作物功能基因组学的一个强有力的模式系统，因为其参考基因组最近已被测序，功能工具正在增加。然而，需要研究细胞和组织内基因功能的新方法来了解细胞和亚细胞水平上的蛋白质功能，这将最终为作物改良提供指导信息。 该项目的目的是在玉米中产生工具，可用于驱动任何基因在一些特定的组织或细胞类型中的表达。这些工具的使用将允许可视化的报告基因与前所未有的空间和时间分辨率，也将使功能基因过表达或基因敲除研究。此外，该项目将利用新改进的荧光蛋白颜色标签来标记亚细胞或发育区室，并将开发用于生长玉米植物的实时成像技术，以证明所有报告基因系在功能研究中的实用性。 因此，该项目将通过提供一种了解发育过程中特定细胞和组织中基因功能的手段，改变玉米的功能遗传实验。该项目将为研究界提供50种组织特异性激活剂/报告基因系、20种荧光标记蛋白系以及完全表征的体内成像方法的稳定转化种子的永久库存。 它还将开发一个处理大型图像数据集的管道，该管道将适用于其他项目。所有资源，包括图像、种子和基因构建体，都将通过项目网站http://maize.jcvi.org/cellgenomics公开提供。为了长期可用，图像将迁移到社区数据库MaizeGDB，种子将存放在玉米遗传库存中心。更广泛的影响。 该项目将把教育和推广结合起来，为小大角学院的美洲土著学生组织一次年度遗传学讲习班，这是一所乌鸦民族的部落学院。 该项目还将通过生成玉米细胞和生长植物的3D交互式动画，显示亚细胞区室和基因表达数据，将功能研究整合到教育工具中。这些动画将由三个目标群体进行beta测试，包括Little Big Horn学院的美洲原住民学生，Gallaudet大学细胞生物学课上的聋人和重听学生，以及怀俄明州大学生物学学生。动画将向公众提供，并纳入参与机构的课程。