MRI: Acquisition of a 900 MHz Ultra-High Field NMR Spectrometer

MRI：购买 900 MHz 超高场核磁共振波谱仪

• 批准号: 0922862
• 负责人: Charles Sanders
• 金额: $ 394.8万
• 依托单位: Vanderbilt University Medical Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922862&HistoricalAwards=false
• 关键词: MRI; Acquisition; 900; MHz; Ultra

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). Funding is provided for installation of a 900 MHz nuclear magnetic resonance (NMR) spectrometer into the Biomolecular NMR Facility at Vanderbilt University. This spectrometer will be used primarily for multidimensional solution NMR studies of the structures and interactions of biomacromolecules and is expected to have a broad impact on scientific research, education, and training at Vanderbilt. In addition, this instrument will be made available to scientists with justified need throughout the mid-south region of the US. The available instrument time for the new 900 will be divided among the five major users (70%), other Vanderbilt users (10%), outside users (15%), and for training (5%). Projects include: (i) Studies of large micellar complexes of integral membrane proteins such as diacylglycerol kinase, the amyloid precursor protein, and G protein coupled receptors, as well as a large soluble receptor-effector proteins, such as arrestins. (ii) Application of solution NMR to protein-protein complexes and multi-domain proteins involved in Ca2+ signaling, protein ubiquitination, and DNA replication, damage response and repair. (iii) Characterization of the detailed chemistry and conformations of DNA adducts formed by reaction of nucleic acids with electrophiles, a major cause of genetic lesions in biology. (iv) Studies of the structures of designed proteins with internal symmetry. (v) Use of NMR to characterize bacterial two-component signaling, including large protein-protein and protein-DNA complexes. The 900 MHz spectrometer will broadly impact research and education by: (i) Enabling major discoveries in structural biology, as well as complementing and extending discoveries being made in other major research initiatives. (ii) Improving the quality of research training for students pursuing advanced degrees. (iii) Enhancing the academic research infrastructure of Vanderbilt University and furthering the academic research infrastructure of the mid-south region of the US. (iv) Attracting students and other trainees, particularly from underrepresented groups and women, to science and engineering. The role of the 900 MHz NMR spectrometer in furthering these goals will be actively promoted through the participation of the users group and NMR Facility staff in a variety of new and existing outreach programs.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。为在范德比尔特大学的生物分子核磁共振设施中安装一台900兆赫核磁共振光谱仪提供了资金。 该光谱仪将主要用于生物大分子结构和相互作用的多维溶液NMR研究，预计将对范德比尔特的科学研究、教育和培训产生广泛影响。 此外，该仪器将提供给美国中南部地区有合理需求的科学家。 新900的可用仪器时间将在五个主要用户（70%）、其他范德比尔特用户（10%）、外部用户（15%）和培训（5%）之间分配。 项目包括：（i）研究整合膜蛋白的大胶束复合物，如二酰基甘油激酶、淀粉样前体蛋白和G蛋白偶联受体，以及大的可溶性受体效应蛋白，如抑制蛋白。 (ii)溶液NMR在蛋白质-蛋白质复合物和涉及Ca 2+信号传导、蛋白质泛素化、DNA复制、损伤反应和修复的多结构域蛋白质中的应用。 (iii)对核酸与亲电试剂反应形成的DNA加合物的详细化学和构象的表征，是生物学中遗传损伤的主要原因。 (iv)具有内部对称性的蛋白质结构设计研究。(v)使用NMR表征细菌双组分信号，包括大蛋白质-蛋白质和蛋白质-DNA复合物。 900 MHz频谱仪将通过以下方式对研究和教育产生广泛影响：（i）促成结构生物学的重大发现，以及补充和扩展其他重大研究举措中的发现。(ii)提高高学历学生科研训练质量。(iii)加强范德比尔特大学的学术研究基础设施，促进美国中南部地区的学术研究基础设施。 (iv)吸引学生和其他受训人员，特别是来自代表性不足的群体和妇女的学生和受训人员学习科学和工程。 900兆赫核磁共振波谱仪在推进这些目标方面的作用将通过用户组和核磁共振设施工作人员参与各种新的和现有的推广方案得到积极促进。