MRI: Acquisition of a 900 MHz Ultra-High Field NMR Spectrometer

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。提供资金用于在范德比尔特大学的生物分子核磁共振设施中安装900 MHz核磁共振（NMR）光谱仪。该光谱仪将主要用于生物大分子结构和相互作用的多维溶液核磁共振研究，预计将对范德比尔特大学的科学研究、教育和培训产生广泛影响。此外，该仪器将提供给整个美国中南部地区有正当需要的科学家。新900的可用仪器时间将分配给五个主要用户（70%），其他范德比尔特用户（10%），外部用户（15%）和培训（5%）。项目包括：(i)研究整体膜蛋白的大型胶束复合物，如二酰基甘油激酶、淀粉样前体蛋白和G蛋白偶联受体，以及大型可溶性受体-效应蛋白，如阻滞蛋白。（ii）溶液NMR在参与Ca2+信号、蛋白泛素化、DNA复制、损伤响应和修复的蛋白-蛋白复合物和多结构域蛋白中的应用。（iii）核酸与亲电试剂反应形成的DNA加合物的详细化学和构象的特征，这是生物学中遗传病变的主要原因。（iv）研究设计的具有内部对称性的蛋白质结构。(v)利用核磁共振表征细菌双组分信号，包括大的蛋白质-蛋白质和蛋白质- dna复合物。900兆赫光谱仪将通过以下方式对研究和教育产生广泛影响：(i)使结构生物学的重大发现成为可能，并补充和扩展其他重大研究计划中的发现。（ii）提高攻读高级学位学生的研究训练质量。（三）加强范德比尔特大学的学术研究基础设施建设，推进美国中南部地区的学术研究基础设施建设。吸引学生和其他受训者，特别是代表性不足的群体和妇女学习科学和工程。通过用户组和核磁共振设施工作人员参与各种新的和现有的外展计划，将积极促进900 MHz核磁共振光谱仪在进一步实现这些目标方面的作用。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). Funding is provided for installation of a 900 MHz nuclear magnetic resonance (NMR) spectrometer into the Biomolecular NMR Facility at Vanderbilt University. This spectrometer will be used primarily for multidimensional solution NMR studies of the structures and interactions of biomacromolecules and is expected to have a broad impact on scientific research, education, and training at Vanderbilt. In addition, this instrument will be made available to scientists with justified need throughout the mid-south region of the US. The available instrument time for the new 900 will be divided among the five major users (70%), other Vanderbilt users (10%), outside users (15%), and for training (5%). Projects include: (i) Studies of large micellar complexes of integral membrane proteins such as diacylglycerol kinase, the amyloid precursor protein, and G protein coupled receptors, as well as a large soluble receptor-effector proteins, such as arrestins. (ii) Application of solution NMR to protein-protein complexes and multi-domain proteins involved in Ca2+ signaling, protein ubiquitination, and DNA replication, damage response and repair. (iii) Characterization of the detailed chemistry and conformations of DNA adducts formed by reaction of nucleic acids with electrophiles, a major cause of genetic lesions in biology. (iv) Studies of the structures of designed proteins with internal symmetry. (v) Use of NMR to characterize bacterial two-component signaling, including large protein-protein and protein-DNA complexes. The 900 MHz spectrometer will broadly impact research and education by: (i) Enabling major discoveries in structural biology, as well as complementing and extending discoveries being made in other major research initiatives. (ii) Improving the quality of research training for students pursuing advanced degrees. (iii) Enhancing the academic research infrastructure of Vanderbilt University and furthering the academic research infrastructure of the mid-south region of the US. (iv) Attracting students and other trainees, particularly from underrepresented groups and women, to science and engineering. The role of the 900 MHz NMR spectrometer in furthering these goals will be actively promoted through the participation of the users group and NMR Facility staff in a variety of new and existing outreach programs.