Acquisition of a Zeiss LSM 900 confocal microscope with Airyscan 2 for an Imaging and Microscopy Core

购买配备 Airyscan 2 的 Zeiss LSM 900 共焦显微镜作为成像和显微镜核心

• 批准号: 10632858
• 负责人: SEAN M WILSON
• 金额: $ 59.37万
• 依托单位: LOMA LINDA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-15 至 2024-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632858
• 关键词: Advisory Committees; Applications Grants; Award; Cancer Biology; Cardiovascular Physiology; Cells; Confocal Microscopy; Contract Services; Core Facility; Development; Dimensions; Fluorescence; Fluorescent Probes; Funding; Image; Imaging technology; In Situ Hybridization; Institution; Label; Lasers; Measures; Medicine; Microscope; Microscopy; Minor; Morphology; Neurobiology; Neuroendocrinology; Pregnancy; Preparation; Proteins; Radiation; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Scanning; Signal Transduction; System; Systems Integration; Tissues; Training Programs; United States National Institutes of Health; Universities; cellular imaging; confocal imaging; experience; health disparity; instrument; instrumentation; interest; light transmission; mosaic; multi-photon; multidisciplinary; operation; programs; protein expression; real-time images; reconstruction; superresolution imaging

Research Summary This grant proposal seeks funding for a Zeiss LSM 900 laser scanning confocal microscope with Airyscan 2 for NIH funded investigators who use the Advanced Imaging and Microscopy Core Facility at Loma Linda University to perform both histochemical analyses and real-time imaging of cells and tissues. On the one hand, the system would be used to localize various proteins and cells using fluorescent labeling approaches, while on the other it would be used to examine the activity of living cells and tissues. These two imaging technologies are required as the NIH funded major and minor user group has diverse needs. Their research encompasses cardiovascular physiology, pregnancy, development, neurobiology, neuroendocrinology, cancer biology, radiation medicine, regenerative medicine, and health disparities, but all have common interests in measuring the spatial apposition between cells, changes in protein expression, localization of proteins within cells, and spatial and temporal sequences involved with cell signaling. The research record of the user group is excellent, representing some of the most successful investigators at the institution. The Zeiss LSM 900 confocal would support numerous NIH research grants and investigative programs across campus and training programs. The core facility has two shared-use confocal microscopes that are near or at their end of functional use. Acquisition of a Zeiss LSM 900 with a AiryScan 2 will solve this pressing issue and will enable funded researchers to continue their studies including cell signaling, in-situ hybridization, protein expression and localization, cell and tissue morphology, interactions between molecules and proteins, spatial reconstruction, spatial-temporal relationships, and subcellular transport. These questions require the ability to image single or multiple fluorescent probes in either fixed or live preparations. Based on the needs of the funded projects, a Zeiss LSM 900 confocal microscope is an ideal replacement instrument. The instrument has 2 high-sensitivity confocal channels for recording fluorescence; one for transmitted light (DIC) and has an Airyscan 2 unit for super-resolution imaging along with adaptive focusing capabilities and can perform mosaic tiling for multidimensional reconstruction. The Airyscan 2 will also allow for rapid confocal imaging for spatial and temporal recordings of cell signaling systems, which are key aspects to multiple awards. The Zeiss LSM 900 offers a fully integrated system available for the wide breadth of studies of the user group and will provide continuity with an existing multiphoton Zeiss 710 system. The institution recognizes the quality of the facility and user group and has strongly supported the facility for over a decade with full funding for staffing, supplies, and service contracts. The PI has considerable experience with confocal microscopy and will oversee the daily operation of the instrument and be part of a multidisciplinary internal advisory committee that oversees the core facility. The instrument will be housed in the existing AIM facility, providing continued contemporary instrumentation for this highly used shared-use imaging core.

研究摘要本拨款申请旨在为蔡司LSM 900激光扫描共聚焦 配备Airyscan 2的显微镜，供NIH资助的使用高级成像和显微镜核心的研究人员使用 洛马琳达大学的设施，可进行组织化学分析和细胞实时成像，并 组织中一方面，该系统将用于使用荧光定位各种蛋白质和细胞 标记方法，而另一方面，它将用于检查活细胞和组织的活性。 这两种成像技术是必需的，因为NIH资助的主要和次要用户组具有不同的 需求他们的研究包括心血管生理学，怀孕，发育，神经生物学， 神经内分泌学，癌症生物学，放射医学，再生医学和健康差异，但所有 在测量细胞之间的空间并置，蛋白质表达的变化， 蛋白质在细胞内的定位，以及与细胞信号传导有关的空间和时间序列。的 用户组的研究记录非常好，代表了一些最成功的研究人员， 机构。蔡司LSM 900共焦显微镜将支持美国国立卫生研究院（NIH）的众多研究经费和调查研究。 校园和培训项目。核心设施有两个共用的共聚焦显微镜 接近或处于其功能使用的末端。购买带有AiryScan 2的蔡司LSM 900将解决这一问题 这是一个紧迫的问题，将使受资助的研究人员能够继续他们的研究，包括细胞信号，原位 杂交、蛋白质表达和定位、细胞和组织形态、分子间的相互作用 和蛋白质，空间重建，时空关系，和亚细胞运输。这些问题 需要在固定或活的制剂中成像单个或多个荧光探针的能力。基于 根据资助项目的需要，蔡司LSM 900共聚焦显微镜是理想的替代仪器。 该仪器有2个高灵敏度共聚焦通道用于记录荧光;一个用于透射光 (DIC)并具有Airyscan 2单元，用于超分辨率成像，沿着自适应聚焦能力， 执行马赛克平铺以进行多维重建。Airyscan 2还将允许快速共聚焦 成像用于细胞信号系统的空间和时间记录，这是多个 奖蔡司LSM 900提供了一个完全集成的系统，可用于对 用户组，并将提供与现有的多光子蔡司710系统的连续性。该机构承认， 设施和用户群的质量，并大力支持该设施超过十年， 为人员配备、供应和服务合同提供资金。PI在共聚焦方面有相当丰富的经验 显微镜，并将监督仪器的日常运作，并成为多学科内部的一部分， 监督核心设施的咨询委员会。该仪器将被安置在现有的AIM设施中， 为这一高度使用的共用成像核心提供持续的现代仪器。