MRI: Acquisition of Instrumentation for Enhancement of Undergraduate Research and Pedagogy in Molecular Bioengineering

MRI：购置仪器以增强分子生物工程本科研究和教学

• 批准号: 0923273
• 负责人: James Ferri
• 金额: $ 35.71万
• 依托单位: Lafayette College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923273&HistoricalAwards=false
• 关键词: MRI; Acquisition; Instrumentation; Enhancement; Undergraduate

0923273Ferri"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."This MRI requests a Cell-Scale Biotester 5000 biaxial materials testing system, Applied Biosystems (ABI) real-time polymerase chain reaction (RT-PCR) analysis suite, and a Nikon Ti-E inverted C1 confocal laser scanning microscope. The Cell-Scale Biotester 5000 will be used to measure mechanical properties of synthetic and natural soft nanomembranes, the ABI RT-PCR system will be used to measure gene expression in response to engineering strain, and the Nikon Ti-E C1 will be used to image cellular adhesion proteins as a function of mechanical properties of the substrate and applied load and molecular transport in engineered nanocomposite tissue scaffolds. The feedback of local matrix stiffness on cellular functions has important implications for development, differentiation, disease, and regeneration. This research will have a significant impact in the emerging technologies in biomedicine and the relationships between mechanical response, biochemical signaling (specifically gene expression), and cell microstructure. The PIs propose an integrative research and education program at Lafayette College, an undergraduate institution, which will continue to expand the participation of undergraduates in molecular biomechanics while building infrastructure for the Bioengineering/Biotechnology program at Lafayette. Support for this MRI will also strengthen the early career development of a faculty member from an underrepresented group (specifically women) previously identified through the NSF-GRFP as a young researcher with outstanding potential, thereby enhancing the continued recruitment and retention of women in engineering at Lafayette.

0923273 Ferri“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。“该MRI需要Cell-Scale Biotester 5000双轴材料测试系统、应用生物系统（ABI）实时聚合酶链反应（RT-PCR）分析套件和Nikon Ti-E倒置C1共聚焦激光扫描显微镜。Cell-Scale Biotester 5000将用于测量合成和天然软纳米膜的机械性能，ABI RT-PCR系统将用于测量工程菌株的基因表达，Nikon Ti-E C1将用于成像细胞粘附蛋白，作为工程纳米复合材料组织支架中基底机械性能和施加负载和分子运输的函数。局部基质刚度对细胞功能的反馈对发育、分化、疾病和再生具有重要意义。这项研究将对生物医学中的新兴技术以及机械响应，生化信号（特别是基因表达）和细胞微观结构之间的关系产生重大影响。PI提出了一个综合研究和教育计划，在拉斐特学院，一个本科院校，这将继续扩大本科生在分子生物力学的参与，同时在拉斐特生物工程/生物技术计划的基础设施建设。支持这个MRI也将加强教师的早期职业发展从一个代表性不足的群体（特别是妇女）以前通过NSF-GRFP确定为一个年轻的研究人员具有杰出的潜力，从而提高继续招聘和妇女在拉法叶工程保留。