Regulation of Early Endosperm Development in Maize

玉米早期胚乳发育的调控

• 批准号: 0923880
• 负责人: Ramin Yadegari
• 金额: $ 493.18万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923880&HistoricalAwards=false
• 关键词: Regulation; Early; Endosperm; Development; Maize

_____________________________________________________________________________________________________________________________________***PI: Ramin Yadegari (University of Arizona) CoPIs: Amy Clore (New College of Florida), Joanne Dannehoffer (Central Michigan University), Gary Drews (University of Utah) and Brian Larkins (University of Nebraska)The goal of this research project is to understand the gene networks that control endosperm development and function in maize. The endosperm of the seed is biologically and economically important. More than 50% of the calories in the human diet originate from cereal endosperm, which also serves as a raw material for manufacturing numerous industrial products. Early endosperm development in maize, the period between 0 and 8 days after pollination, encompasses the time when the endosperm grows from a single cell into a multicellular structure composed of different cell types. Although the molecular mechanisms that control this period of development in maize have not been elucidated, they are likely to affect many important processes, including embryo nutrition, starch and storage protein accumulation during grain filling, and seed maturation. In this project a network of maize transcription factors expressed during early endosperm development will be identified, and their temporal and spatial expression patterns will be determined. The function of a subset of these genes will be characterized in maize and Arabidopsis plants. In addition, the cytological patterns of early endosperm development will be described in a diverse set of maize inbred lines. These inbreds are currently being genetically and phenotypically characterized by a large number of laboratories, and these data will make it possible to evaluate the importance of the identified genes to a variety of grain quality traits. The broader educational impacts of this project will enhance the infrastructure of research and training at all the participating institutions through the continued instruction of undergraduate and graduate students and training of postdoctoral researchers. A significant component of the proposed research will involve faculty members at institutions with predominately (Central Michigan University CMU) or exclusively (New College of Florida NCF) undergraduate populations. Student researchers from CMU and NCF will have an opportunity to spend a summer each year at the University of Arizona and the University of Utah for further training and education. Undergraduate researchers will be recruited from the classroom, local community colleges, and underrepresented student populations at all institutions. In addition, an important goal of the project is to increase the representation of underrepresented minorities in science and to communicate science through outreach to K-12 teachers and to nonscientists. The scientific and educational outcomes of this project will be disseminated through the project website http://cals.arizona.edu/earlyendosperm and Gramene (http://www.gramene.org/).

_____________________________________________________________________________________________________________________________________*** PI:白木Yadegari(亚利桑那大学)CoPIs:艾米克罗尔(新佛罗里达大学),乔安妮Dannehoffer(中央密歇根大学)、加里·德鲁(犹他大学)和布莱恩拉金斯(内布拉斯加州大学)这个研究项目的目标是理解基因网络,控制在玉米胚乳发育和功能。种子的胚乳在生物学和经济上都很重要。人类饮食中超过50%的卡路里来自谷物胚乳，它也是制造许多工业产品的原料。玉米的早期胚乳发育，即授粉后0 ~ 8天，包括胚乳从单细胞发育成由不同细胞类型组成的多细胞结构的时间。尽管控制玉米这一发育时期的分子机制尚未阐明，但它们可能影响许多重要的过程，包括胚胎营养、籽粒灌浆过程中淀粉和储存蛋白的积累以及种子成熟。本项目将鉴定玉米胚乳早期发育过程中表达的转录因子网络，并确定其时空表达模式。这些基因的一个子集的功能将在玉米和拟南芥植物中得到表征。此外，早期胚乳发育的细胞学模式将描述在一个多样化的玉米自交系。这些自交系目前正在大量的实验室进行遗传和表型表征，这些数据将使评估鉴定的基因对各种粮食品质性状的重要性成为可能。该项目更广泛的教育影响将通过继续指导本科生和研究生以及培训博士后研究人员，加强所有参与机构的研究和培训基础设施。拟议研究的一个重要组成部分将涉及主要由中密歇根大学（CMU）或专门由佛罗里达新学院（NCF）本科生组成的机构的教职员工。CMU和NCF的学生研究人员每年将有机会在亚利桑那大学和犹他大学度过一个夏天，接受进一步的培训和教育。本科生研究人员将从课堂、当地社区学院和所有机构中代表性不足的学生群体中招募。此外，该项目的一个重要目标是增加代表性不足的少数民族在科学领域的代表性，并通过与K-12教师和非科学家的接触来传播科学。本项目的科学和教育成果将通过项目网站http://cals.arizona.edu/earlyendosperm和Gramene （http://www.gramene.org/）发布。

项目成果

Temporal patterns of gene expression in developing maize endosperm identified through transcriptome sequencing

• DOI: 10.1073/pnas.1406383111
• 发表时间: 2014-05-27
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Li, Guosheng;Wang, Dongfang;Yadegari, Ramin
• 通讯作者: Yadegari, Ramin