Nanoparticle-Bridge DNA Sensor for Electrical Detection of Ultra-Low Concentrations of DNA Molecules

用于超低浓度 DNA 分子电检测的纳米颗粒桥 DNA 传感器

• 批准号: 0925997
• 负责人: Seong Jin Koh
• 金额: $ 31.96万
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0925997&HistoricalAwards=false
• 关键词: Nanoparticle; Bridge; DNA; Sensor; Electrical

Proposal 0925997PI: Seong Jin KohThe University of Texas at ArlingtonIntellectual Merit:This project will investigate a new DNA sensing method which is capable of detecting ultra-low concentrations of DNA molecules without any amplification. Importantly, its sensor output will be a direct electrical signal, without using any transducers, which has numerous benefits including compactness, portability, facile integration with other systems, multiplex sensing, and low cost. The key components of the proposed DNA sensor are 1) DNA hybridization is carried out exclusively on the surface of nanoparticles (~50 nm in diameter), 2) the presence of target DNA (to-be-detected DNA) molecules leads to the formation of nanoparticle-DNA-nanoparticle conjugates, and 3) the nanoparticle-DNA-nanoparticle conjugates are detected using simple I-V measurements. This approach allows the detection of extremely small concentrations of DNA molecules since, in principle, the formation of even a single nanoparticle-DNA-nanoparticle conjugate can lead to a current flow across electrodes. The high sensitivity of the proposed DNA sensing method could play a significant role in advancing biomedical science/technology, such as disease diagnosis and medicine development, as well as protecting our citizens and soldiers from biological threats/attacks. Broader Impacts:The broader impacts of this project lie in the educational and outreach programs that will be integrated with its research component. Student training is one of the important parts of the educational program. By performing the proposed research, students will be extensively trained as competitive scientists and engineers. This will occur through their designing the experiments, training on cutting-edge experimental tools, obtaining deep and broad knowledge of the field through extensive literature search and reading, discussing the experimental results with the PI and colleagues, and presenting their findings in international conferences and peer-reviewed journals. The other important educational component is in the classroom. The course MSE5351 (Current Topics in Nanotechnology) will provide students in-depth knowledge of recent important advancements in the field of nanotechnology. This will be done through PI's lecturing on key concepts and techniques in nanoscale science and technology and also students' reading of important landmark publications and discussing them in the class. In addition, this class will provide several experimental modules which will take place in the form of demonstrating key experimental steps and/or carrying out hands-on experiments. Importantly, the resulting findings from the proposed research will be incorporated into the experimental modules. This program will also run outreach programs by providing science camps for K-12 students and teachers. In particular, one of the camps will be targeted for elementary students in their 4th or 5th grades. By focusing on young ages, this camp aims to expose young minds to the exciting and fun world of science and technology and to inspire them to pursue careers in science and technology, ultimately contributing to preparing for our nation's superiority in science and technology in generations to come.

提案0925997 PI：Seong Jin KohThe University of Texas at Arlington智力优势：该项目将研究一种新的DNA传感方法，该方法能够在没有任何扩增的情况下检测超低浓度的DNA分子。重要的是，它的传感器输出将是直接的电信号，而不使用任何换能器，这具有许多优点，包括紧凑性，便携性，易于与其他系统集成，多路传感和低成本。所提出的DNA传感器的关键组件是1）DNA杂交仅在纳米颗粒（直径约50 nm）的表面上进行，2）靶DNA（待检测DNA）分子的存在导致纳米颗粒-DNA-纳米颗粒缀合物的形成，以及3）使用简单的I-V测量来检测纳米颗粒-DNA-纳米颗粒缀合物。这种方法允许检测极小浓度的DNA分子，因为原则上，即使单个纳米颗粒-DNA-纳米颗粒缀合物的形成也可以导致电流流过电极。所提出的DNA传感方法的高灵敏度可以在推进生物医学科学/技术（例如疾病诊断和医学开发）以及保护我们的公民和士兵免受生物威胁/攻击方面发挥重要作用。更广泛的影响：该项目的更广泛的影响在于将与其研究组成部分相结合的教育和推广计划。学生培训是教育计划的重要组成部分之一。通过执行拟议的研究，学生将被广泛培训为有竞争力的科学家和工程师。这将通过他们设计实验，培训尖端的实验工具，通过广泛的文献检索和阅读获得该领域的深入和广泛的知识，与PI和同事讨论实验结果，并在国际会议和同行评审期刊上展示他们的发现。另一个重要的教育环节是在课堂上。课程MSE 5351（当前纳米技术主题）将为学生提供有关纳米技术领域最近重要进展的深入知识。这将通过PI对纳米科学技术中关键概念和技术的讲座以及学生阅读重要的标志性出版物并在课堂上讨论来完成。此外，本课程将提供几个实验模块，这些模块将以演示关键实验步骤和/或进行动手实验的形式进行。重要的是，拟议研究的结果将纳入实验模块。该计划还将通过为K-12学生和教师提供科学营地来开展外展计划。特别是，其中一个营地将针对小学四年级或五年级的学生。通过关注年轻人，这个夏令营旨在让年轻人接触到令人兴奋和有趣的科学技术世界，并激励他们追求科学技术事业，最终为我们国家在未来几代人中的科学技术优势做好准备。