Molecular and Genetic Analysis of Petal Growth in Arabidopsis

拟南芥花瓣生长的分子和遗传分析

• 批准号: 0926347
• 负责人: Thomas Jack
• 金额: $ 62.02万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0926347&HistoricalAwards=false
• 关键词: Molecular; Genetic; Analysis; Petal; Growth

This project is focused on understanding how plant organs grow and attain their final shape. In particular, this work is focused on the growth of petals in the plant Arabidopsis thaliana. Petals are excellent organs for studies such as these because they are accessible and are not essential for plant reproduction. In particular, this project will examine the role of two genes that function to control petal growth. The first gene is a micro-RNA gene (miR319a) that functions by regulating a set of DNA binding transcription factor genes of the TCP family. The second gene, named DORNRÖSCHEN-LIKE (DRNL), is a DNA binding transcription factor. When either the miR319a or DRNL gene is inactivated, the petals develop, but do not attain the normal shape. However, when both miR319a and DRNL are inactivated together, the petals are reduced to very small filamentous structures. The work in this project is focused on understanding how the genetic and molecular pathways controlled by miR319a and DRNL function together to control the growth of petals. This project has several broader impacts. First, this work is important because it will contribute to our knowledge about how plant organs attain their shape. A more detailed understanding of plant growth in petals will increase our knowledge of the growth of more agriculturally important plant organs such as leaves, fruits, and seeds, three key components of the human diet. Second, this project will provide training for several graduate students and undergraduate students. Third, the primary outreach activity of this project involves participation in a summer professional development workshop for high school teachers from Vermont and New Hampshire, many of whom are from under-resourced public schools.

该项目的重点是了解植物器官如何生长并达到最终形状。 特别是，这项工作的重点是在植物拟南芥花瓣的生长。 花瓣是研究这些的极好器官，因为它们是可接近的，并且不是植物繁殖所必需的。 特别是，该项目将研究两个基因的作用，控制花瓣的生长。 第一个基因是微小RNA基因（miR 319 a），其通过调节TCP家族的一组DNA结合转录因子基因发挥功能。 第二个基因，命名为DORNRÖSCHEN-LIKE（DRNL），是一种DNA结合转录因子。 当miR 319 a或DRNL基因失活时，花瓣发育，但不能达到正常形状。 然而，当miR 319 a和DRNL两者一起失活时，花瓣减少到非常小的丝状结构。 该项目的工作重点是了解miR 319 a和DRNL控制的遗传和分子途径如何共同控制花瓣的生长。 这个项目有几个更广泛的影响。首先，这项工作很重要，因为它将有助于我们了解植物器官是如何形成它们的形状的。 更详细地了解植物在花瓣中的生长将增加我们对农业上更重要的植物器官生长的了解，如叶子，果实和种子，这是人类饮食的三个关键组成部分。 第二，本项目将为几名研究生和本科生提供培训。 第三，该项目的主要外联活动包括参加为来自佛蒙特州和新罕布什尔州的高中教师举办的夏季专业发展讲习班，其中许多教师来自资源不足的公立学校。