The Development of Microbial Associations in Major Reef Building Corals of the Pacific Ocean
太平洋主要造礁珊瑚微生物群落的发展
基本信息
- 批准号:0928806
- 负责人:
- 金额:$ 42.89万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-10-01 至 2014-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Fundamental to the study of coral-microbial associations is an understanding of when and how the relationships are established, and their specificity. Recently, the investigators provided the first evidence of a specific association between an early life history stage of a coral (Pocillopora meandrina) and a particular group of bacteria (Jannaschia lineage of the Roseobacter clade of Alphaproteobacteria). They will be expanding this work by examining the onset of microbial associations in key reef building corals from Hawaii in the North Pacific Ocean and Moorea, French Polynesia, in the South Pacific Ocean. Understanding the onset, specificity and function of the microbial community associated with these coral species is necessary to understand and predict the coral holobiont response to a changing environment.The main objectives of this proposal are to: 1. Use cultivation independent techniques to identify and quantify microorganisms associated with several major reef building corals of Hawaii in the North Pacific Ocean that represent a variety of reproductive strategies (brooding and broadcast spawning) and differing modes of zooxanthellae symbiont transmission (vertical vs. horizontal), throughout the reproductive cycle, early developmental stages, and post-settlement stages of each.2. Collect and analyze similar samples from the same (or similar) species of coral found in the South Pacific Ocean in Moorea, French Polynesia, in order to assess whether the associations documented in objective 1 are localized to Hawaii, or broadly distributed across the Pacific and likely to represent common features of coral development.3. Use fluorescence in situ hybridization to enumerate cells of the Jannaschia lineage of the Roseobacter clade throughout the development cycle of P. meandrina collected in Hawaii. The PIs will expand this objective to include other coral species, target bacteria, and/or geographic location as they identify additional associations.4. Isolate microorganisms prevalent in cultivation-independent surveys of P. meandrina-associated microbial communities (e.g. Jannaschia sp.) by the application of novel culturing techniques, in order to develop model systems for the investigation of coral-microbe interactions.Intellectual merit Coral reefs are in decline as a result of increasing environmental stress due to anthropogenic activity, and there is now considerable evidence indicating that they are under threat from the effects of rising sea surface temperature and ocean acidification. Microorganisms associated with corals are thought to play a variety of potentially important roles in maintaining the health and resiliency of the coral host, and advances in methodology primarily driven by developments in the field of molecular biology are facilitating growing insight into this association. Much of coral-microbial research is focused on the contribution of microorganisms to disease and bleaching, and is focused almost exclusively on adult coral colonies. This study will provide unique information on the manner in which microorganisms interact with healthy corals throughout their developmental cycle, the specificity of these relationships, how they are initiated, and their distribution and frequency in nature.Broader impacts (1) This proposal will support the training of one postdoctoral scholar in advancing molecular microbial ecology techniques, novel cultivation-based methodology, and coral biology. (2) The investigators will develop a website that will contain information on coral-microbial interactions, with content designed to serve as an educational resource for K-12 students, and to serve as a resource for other scientists interested in coral-microbial associations. Data generated from this study, including publications, presentations, microbial strains, gene sequences, photomicrographs and phylogenetic analyses, will be made publicly available via the website. (3) The postdoc and PI will design and annually offer a five-day intensive training workshop on the use of ARB sequence analysis program, including the application of fluorescence in situ microscopy to marine bacteria and coral samples. (4) The proposal will support the training of undergraduate students under the mentorship of the postdoctoral scholar, and will take advantage of existing programs and relationships to specifically offer these opportunities to underrepresented students at the University of Hawaii such as Native Hawaiians and Pacific Islanders.
珊瑚微生物协会的研究的基础是了解何时以及如何建立关系,以及它们的特异性。最近,研究人员提供了珊瑚(Pocillopora meandrina)的早期生活史阶段与一组特定细菌(Alphaproteobacteria的Roseopora分支的Jannaschia谱系)之间特定关联的第一个证据。他们将通过研究来自北太平洋夏威夷和南太平洋法属波利尼西亚莫雷阿的关键造礁珊瑚中微生物协会的开始来扩大这项工作。了解与这些珊瑚物种相关的微生物群落的发生、特异性和功能,对于了解和预测珊瑚全生生物对不断变化的环境的反应是必要的。使用培养独立的技术,以确定和量化微生物与几个主要的造礁珊瑚的夏威夷在北太平洋,代表了各种生殖策略(育雏和广播产卵)和不同模式的虫黄藻共生体传输(垂直与水平),在整个生殖周期,早期发育阶段,和后定居阶段的每一个。收集和分析在南太平洋法属波利尼西亚莫雷阿发现的相同(或类似)珊瑚物种的类似样本,以评估目标1中记录的相关性是局限于夏威夷,还是广泛分布于整个太平洋,并可能代表珊瑚发育的共同特征。在夏威夷采集的P. meandrina的整个发育周期中,使用荧光原位杂交对蔷薇属进化枝的Jannaschia谱系细胞进行计数。PI将扩大这一目标,以包括其他珊瑚物种,目标细菌和/或地理位置,因为他们确定了其他关联。分离出在不依赖培养的Meandrina相关微生物群落调查中普遍存在的微生物(例如Jannaschia sp.)由于人类活动对环境造成的压力越来越大,珊瑚礁正在衰退,现在有相当多的证据表明,它们正受到海面温度上升和海洋酸化的威胁。与珊瑚有关的微生物被认为在维持珊瑚宿主的健康和复原力方面发挥着各种潜在的重要作用,主要由分子生物学领域的发展推动的方法学的进步正在促进对这种关联的日益深入的了解。许多珊瑚微生物研究都集中在微生物对疾病和漂白的贡献上,而且几乎完全集中在成年珊瑚群上。本研究将提供关于微生物在整个发育周期中与健康珊瑚相互作用的方式、这些关系的特异性、它们是如何启动的以及它们在自然界中的分布和频率的独特信息。更广泛的影响(1)本提案将支持培养一名博士后学者,推进分子微生物生态学技术、基于培养的新方法和珊瑚生物学。(2)研究人员将开发一个网站,其中包含有关珊瑚-微生物相互作用的信息,其内容旨在作为K-12学生的教育资源,并作为对珊瑚-微生物协会感兴趣的其他科学家的资源。本研究产生的数据,包括出版物、演示文稿、微生物菌株、基因序列、显微照片和系统发育分析,将通过网站公开提供。(3)博士后和PI将设计并每年提供为期五天的关于ARB序列分析程序使用的强化培训研讨会,包括将荧光原位显微镜应用于海洋细菌和珊瑚样本。(4)该提案将支持在博士后学者的指导下对本科生进行培训,并将利用现有的计划和关系,专门为夏威夷大学代表性不足的学生提供这些机会,如夏威夷土著人和太平洋岛民。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael Rappe其他文献
Michael Rappe的其他文献
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{{ truncateString('Michael Rappe', 18)}}的其他基金
Illuminating the physiology, cellular characteristics, and ecogenomics of the first cultivated strain of the SAR86 lineage
阐明 SAR86 谱系第一个栽培菌株的生理学、细胞特征和生态基因组学
- 批准号:
2149128 - 财政年份:2022
- 资助金额:
$ 42.89万 - 项目类别:
Standard Grant
Collaborative Research: Illuminating microbes and their viruses within the dark ocean crust through strain-level approaches
合作研究:通过菌株水平的方法阐明黑暗海洋地壳内的微生物及其病毒
- 批准号:
1851582 - 财政年份:2019
- 资助金额:
$ 42.89万 - 项目类别:
Standard Grant
Population genomics and ecotypic divergence in the most dominant lineage of marine bacteria
海洋细菌最主要谱系的群体基因组学和生态型分化
- 批准号:
1538628 - 财政年份:2015
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$ 42.89万 - 项目类别:
Standard Grant
Collaborative Research: Completing single- and cross-hole hydrgeologic and microbial experiments: Juan de Fuca Flank
合作研究:完成单孔和跨孔水文地质和微生物实验:Juan de Fuca Flank
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1260723 - 财政年份:2013
- 资助金额:
$ 42.89万 - 项目类别:
Continuing Grant
Collaborative Proposal: Characterizing Microbial Activity and Diversity in Hawaiian Basalt Aquifers
合作提案:表征夏威夷玄武岩含水层的微生物活动和多样性
- 批准号:
0527135 - 财政年份:2005
- 资助金额:
$ 42.89万 - 项目类别:
Standard Grant
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