Liquid Membranes in Nanopores with Strip Dispersion for Antibiotic Recovery

纳米孔中的液膜带条分散用于抗生素回收

• 批准号: 0932511
• 负责人: Winston Ho
• 金额: $ 20.56万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0932511&HistoricalAwards=false
• 关键词: Liquid; Membranes; Nanopores; Strip; Dispersion

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). 0932511HoRecent advances in enzymatic synthetic methods using biocatalysts have lead to environmentally friendly production methods of beta-lactam antibiotics. These methods have been limited in their industrial use due to the difficult separation of the desired antibiotic from other components of the reaction mixture. The development of a simple and easy to operate separation process for antibiotic recovery from aqueous solutions and fermentation broths would eliminate a major drawback of enzymatic antibiotic production. The research proposed will focus on the recovery of antibiotics from fermentation broths using supported liquid membranes with strip dispersion. The proposed process will recover and concentrate antibiotics in a simple manner, while providing long-term stability, which is a requirement for industrial use. This process combines extraction and back-extraction, carried out in 2 separate steps in conventional extraction processes, into a one-step membrane process. This one step process not only simplifies the separation process, but also eliminates the needs for the extractor and the back extractor. This process also overcomes the thermodynamic solubility limitation of solvent. Thus, this process will have both significant capital and energy savings. The recovery of Cephalexin will be studied extensively on a laboratory scale to determine important factors in the separation process. The understanding of the mass transfer mechanism involving interfacial complexation reactions and complex diffusion and the development of mathematical models that describe the mass transfer suitable for scale-up are also goals of this work. Once the recovery of Cephalexin is fully understood, similar processes for other antibiotics will be developed. Supported liquid membranes with strip dispersion have never been used in comprehensive studies involving the recovery of antibiotics or biochemicals. The proposed separation scheme provides an efficient and stable separation process. Long-term stability is the key obstacle in commercialization of liquid membrane processes. This research not only is of a great scientific interest in the fundamental understanding of the transport mechanism but also may provide an improved separation scheme of significant technological importance. We believe that it represents a significant contribution to expanding the scientific knowledge and understanding in the science and technology of antibiotic separation/recovery. This work also provides the education of the students involved with such kind of research. The resulting work will have immediate impacts on the industrial production of antibiotics. The proposed one-step process will save energy, reduce costs, eliminate toxic solvents, and operate at higher efficiencies compared to current industrial processes used for antibiotic separation. These improvements will foster the industrial use of enzymatic synthetic methods for antibiotic production. This process also has potential for the effective separation of other organic acids from fermentation broths such as lactic acid, citric acid, and propionic acid and for the recovery of other organic compounds including bio ethanol and butanol.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。使用生物催化剂的酶促合成方法的最新进展导致了环境友好的β-内酰胺抗生素生产方法。这些方法在工业应用中受到限制，因为难以从反应混合物的其它组分中分离所需的抗生素。从水溶液和发酵液中回收抗生素的简单和易于操作的分离方法的开发将消除酶促抗生素生产的主要缺点。本研究将着重于利用支撑液膜分离技术从发酵液中回收抗生素。所提出的工艺将以简单的方式回收和浓缩抗生素，同时提供长期稳定性，这是工业使用的要求。该工艺将传统萃取工艺中的萃取和反萃取两个单独步骤合并为一步膜工艺。这种一步法不仅简化了分离过程，而且消除了对萃取器和反萃取器的需要。该方法还克服了溶剂的热力学溶解度限制。因此，该方法将具有显著的资本和能量节省。将在实验室规模上广泛研究头氨肽的回收率，以确定分离过程中的重要因素。了解的传质机制，包括界面络合反应和复杂的扩散和发展的数学模型，描述适合放大的传质也是这项工作的目标。一旦完全了解头孢氨苄的回收情况，将开发其他抗生素的类似过程。具有剥离分散的支撑液膜从未被用于涉及回收抗生素或生物化学品的综合研究。所提出的分离方案提供了有效且稳定的分离过程。长期稳定性是液膜工艺商业化的关键障碍。这项研究不仅是一个伟大的科学兴趣的运输机制的基本理解，但也可能提供一个改进的分离方案具有重大的技术意义。我们认为，这是对扩大科学知识和理解抗生素分离/回收科学技术的重大贡献。这项工作也提供了参与这种研究的学生的教育。由此产生的工作将对抗生素的工业生产产生直接影响。与目前用于抗生素分离的工业工艺相比，所提出的一步法将节省能源，降低成本，消除有毒溶剂，并以更高的效率操作。这些改进将促进抗生素生产的酶合成方法的工业应用。该方法还具有从发酵液中有效分离其他有机酸（如乳酸、柠檬酸和丙酸）和回收其他有机化合物（包括生物乙醇和丁醇）的潜力。