SBIR Phase I: Fluorescence-Amplified Nana-Assembly for Sensing Bio-Toxins

SBIR 第一阶段：用于传感生物毒素的荧光放大纳米组装体

• 批准号: 0232277
• 负责人: Winston Ho
• 金额: $ 9.99万
• 依托单位: MAXWELL SENSORS INC.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2003-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0232277&HistoricalAwards=false
• 关键词: SBIR; Phase; Fluorescence; Amplified; Nana

This Small Business Innovation Research (SBIR) Phase I project is to develop a novel functional nanostructure for detection/identification of biological warfare agents (BWA). A new class of fluorescence-amplified nano-assembly (FLAN) is proposed for real-time, selective, and ultra-sensitive BWAs and toxins assays. The basic concept of this technology is to mimic the cell membrane under certain organisms and toxins initially attack. Living cells quickly recognize and selectively respond towards invasion. The FLAN using three key elements for target detection: 1) molecular recognition, 2) fluorescence transduction, and 3) fluorescence amplification, to provide simple and direct fluorescent assay. The molecular receptors recognize the BWA; the binding causes a change in the local ternary structure, and which leads to an amplified fluorescent structure that can be quantified optically. The synthetic nanostructures exhibit bioactivities and high stability. The BWA-FLAN receptor binding is a rapid one-step reaction; it does not require complicate separation and washing steps, labeled fluorophore, or visualization reagents. During the Phase I project, the investigator will design and synthesize functional nanostructures with BWA-specific receptors, develop FLAN molecular assembly, develop fluorescence sensing system, characterize, test, and evaluate its technical merits. Highly selective and sensitive molecular recognition is important throughout biology, biotechnology, and clinical diagnostics. COMMERCIAL APPLICATIONS Homeland and civilian defense applications include medical diagnostic of pathogens and diseases as well as non-medical contamination avoidance sensors for biological terrorism agents. The proposed nanostructure-based assays could be easily adapted to targets of interest to the medical community, environmental and agricultural testing, and food industry and used in conjunction with the portable reader for diagnostics in clinical or hospital setting.

这项小型企业创新研究(SBIR)第一阶段项目旨在开发一种新的功能纳米结构，用于检测/识别生物战剂(BWA)。提出了一种新的荧光放大纳米组装技术(FRAN)，用于实时、选择性、超灵敏的生物化学和毒素分析。这项技术的基本概念是模拟某些有机体和毒素最初攻击下的细胞膜。活细胞迅速识别并选择性地对入侵做出反应。FRAN使用三个关键元件进行靶标检测：1)分子识别，2)荧光转导，3)荧光扩增，提供简单直接的荧光分析。分子受体识别BWA；结合导致局部三元结构的变化，从而导致可以光学定量的放大的荧光结构。合成的纳米结构具有生物活性和较高的稳定性。BWA-FRAN受体结合是一个快速的一步反应；它不需要复杂的分离和洗涤步骤、标记的荧光团或显影试剂。在第一阶段项目中，研究人员将设计和合成具有BWA特异性受体的功能纳米结构，开发FRAN分子组装，开发荧光传感系统，表征、测试和评估其技术优势。高度选择性和灵敏的分子识别在生物学、生物技术和临床诊断学中都是重要的。国土和民防的商业应用包括病原体和疾病的医疗诊断，以及用于生物恐怖主义制剂的非医疗污染避免传感器。拟议的基于纳米结构的分析可以很容易地适应医学界、环境和农业测试以及食品行业感兴趣的目标，并与便携式读取器一起用于临床或医院环境中的诊断。