Electroactivated Peptides for Dynamic Functionalization

该奖项由2009年美国复苏和再投资法案(公法111-5)资助。0932989GowerNSF生物传感/CBET计划颁发的这一奖项支持佛罗里达大学材料工程师劳里·高尔和大卫·诺顿教授探索电活性多肽新生物扫描方法的可行性。噬菌体展示组合系统将被用来筛选具有可逆结合性质的无机结合多肽，这样电场就可以用来触发多肽从电子材料的表面吸附和/或解吸。然后，这些多肽可以作为连接物连接其他感兴趣的组件，例如用于生物传感器阵列的生物受体，或用于芯片实验室配置的纳米颗粒“货物”。这里提出的实验将为这种未来的应用奠定基础，首先探索不同的电子配置，以确定哪种刺激模式可以提供最佳的可逆性，而不会对多肽或附着的生物成分产生不利影响。虽然“分子仿生学”方法已经被考虑用于多组分系统的开发，其中可以筛选出与不同无机材料具有选择性结合亲和力的多肽连接物，但这里正在探索的系统将把这项技术带到一个全新的水平，因为它可以提供一种动态绘制多组分表面图案的方法。静态微图案化表面已经为生物技术领域带来了重大进步；能够对表面的功能提供空间和时间控制的能力可能是生物纳米技术的下一个革命性进步。对于下一代XYZ-Microsystem，希望利用微电子学的小尺寸和高效率，以及生物系统提供的高灵敏度和高选择性。最大的挑战是将非电子元件(如生物感受器)与电子转导元件相结合。这里提出的工作不仅将提供有机-无机界面之间的联系，而且将为真正的智能系统提供一种响应设备本身的联系，这些系统根据命令进行组装。人们可以设想实时改变细胞接触引导的表面图案，或生物分子航天飞机的定向聚合轨迹(例如，在智能灰尘生物传感器中)。另一个令人兴奋的进步可能产生于电激活的多肽是设备表面的自清洁能力。这可以实现持续的生物传感，其中可以设计一个流过系统，触发释放堵塞的受体，然后用新的受体替换，这些新的受体会自我组装到失活的表面上。因此，这些探索性研究将为一项全新的技术奠定基础，这项技术可能导致从军事到私营医疗保健部门的可商业化应用。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).0932989GowerThis NSF award by the Biosensing/CBET program supports work by Professors Laurie Gower and David Norton, Materials Engineers at the University of Florida, to explore the feasibility of a new biopanning approach for electroactive peptides. The phage display combinatorial system will be used to screen for inorganic-binding peptides that have reversible binding properties so that an electric field can be used to trigger the sorption and/or desorption of the peptides from an electronic material's surface. These peptides can then serve as linkers to attach other components of interest, such as bioreceptors for biosensor arrays, or nanoparticle "cargo" for lab-on-a-chip configurations. The experiments proposed here will lay the groundwork for such future applications, by first exploring different electronic configurations to determine which mode of stimulus can provide the best reversibility without adversely affecting the peptide or attached biocomponent. While the "molecular biomimetics" approach is already being considered for the development of multicomponent systems, where peptide linkers can be screened to have selective binding affinity to different inorganic materials, the system being explored here will bring this technology to a whole new level because it could provide a means for dynamically patterning a multicomponent surface. Static micropatterned surfaces have already brought significant advances to the biotechnology arena; the ability to provide both spatial and temporal control over a surface's functionality could be the next revolutionary advance for bionanotechnology. For the next generation of XYZ-microsystems, it is desirable to capitalize on the small size and high efficiency of microelectronics, in combination with the high sensitivity and selectivity provided by biological systems. The grand challenge is in integrating non-electronic components (such as bioreceptors) with the electronic transduction elements. The work proposed here will provide not only a linkage between the organic-inorganic interface, but a linkage that is responsive to the device itself, for truly smart systems that assemble on command. One could envision changing a surface pattern in real time for contact guidance of cells, or directed polymerization of tracks for biomolecular shuttles (e.g. in smart dust biosensors). Another exciting advance that could result from electroactivated peptides is the self-cleaning capability of device surfaces. This could enable continuous biosensing, where a flow-through system could be designed that is triggered to release clogged receptors, followed by replacement with fresh receptors which self-assemble onto the de-activated surface. Thus, these exploratory studies will lay the groundwork for a whole new technology, which could lead to commercializable applications ranging from the military to private health care sectors.