DDEP: U.S.-Finland: Tracking Echovirus I Early Entry into Host Cells

0935271Huser这项对国际博士论文增强项目（DDEP）的支持将使加州大学戴维斯分校的生物物理学研究生Gregory McNerney能够前往芬兰与专家合作研究Echovirus（EV1），一种人类病原体病毒。 目的是与芬兰一个高度重视的团队一起研究EV1的进入，该团队由伊瓦斯基拉大学的Varpu Marjomaki博士领导，他研究了调节内吞膜运输的分子机制。 此外，她和她所在机构的同事是模型系统、分析软件和光学探针的创新开发者。在他的芬兰东道主？麦克纳尼先生，你在实验室吗？的计划包括通过开源软件平台BioImageXD测试光学成像分析工具，该平台将用于获得关于共定位，尺寸测量和扩散速率的新数据。因此，DDEP将为美国学生提供理想的，早期的职业机会，以领先的欧洲病毒学专业知识，技术和工具。 此外，该项目预计将巩固Marjomaki博士？的研究小组和麦克纳尼的？的物理学家顾问，加州大学戴维斯分校的托马斯胡瑟博士，他的研究小组致力于高速和高分辨率活细胞成像的先进方法。通过参与芬兰团队？的BioImageXD功能和软件的改进？的鲁棒性，麦克纳尼先生应该获得新的见解细胞摄取途径，同时使用EV1作为模型病毒。 如果成功，联合项目的结果和改进的图像分析技术可以使更广泛的研究群体受益，包括其他生物学学生和对EV1样感染和自我复制感兴趣的医学研究人员。

0935271HuserThis support for an international doctoral dissertation enhancement project (DDEP) will enable biophysics graduate student, Gregory McNerney from the University of California-Davis, to travel to Finland for cooperative work with experts on imaging studies of Echovirus (EV1), a human pathogen virus. The purpose is to study EV1 entry with a highly regarded team in Finland, headed by Dr. Varpu Marjomaki at the University of Jyvaskyla, who has studied the molecular mechanisms that regulate endocytic membrane traffic. Furthermore, she and colleagues at her institution are innovative developers of model systems, analysis software, and optical probes. While at his Finnish host?s laboratory, Mr. McNerney?s plans include the testing of analysis tools for optical imaging via the open-source software platform BioImageXD which will be used to obtain new data on colocalization, size measurements and diffusion rates. Thus, the DDEP will provide the U.S. student with desirable, early career access to leading European virology expertise, techniques, and tools. Furthermore, the project is expected to solidify a promising collaboration between Dr. Marjomaki?s research group and that of McNerney?s physicist advisor, Dr. Thomas Huser at UC- Davis, whose research group works on advanced approaches to live cell imaging at high speed and high resolution.Through participation in the Finnish team?s refinement of BioImageXD functions and the software?s robustness, Mr. McNerney should gain new insights about cellular uptake pathways while using EV1 as a model virus. If successful, results from the joint project and improved image analysis techniques could benefit the broader research community, including other biology students and medical investigators who have interest in EV1-like infections and self-replication.