IDR: Rapid, three-dimensional microscopy of cell-cell interactions in suspension

IDR：悬浮液中细胞间相互作用的快速三维显微镜检查

• 批准号: 1014838
• 负责人: Thomas Huser
• 金额: $ 60.56万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1014838&HistoricalAwards=false
• 关键词: IDR; Rapid; three; dimensional; microscopy

1014838HuserThis project will develop a unique state-of-the-art technology to obtain rapid three-dimensional maps of the function and structure of an extremely important class of cells: immune cells. It will utilize rapid optical imaging at high spatial and temporal resolution of suspension cells in their most natural environment (i.e. freely suspended without substrates present). This new instrument will allow one to measure and record physiological changes in cells in 3 dimensions at millisecond time scales. This system will lead to significant advancement in multiple scientific disciplines, i.e. cellular imaging, cell biology, immunology, virology, etc. by enabling the quantitative assessment of cellular organization and reorganization due to changes in their environment at the sub-cellular level in real-time. This novel instrumentation will significantly enhance the ability to quantify rapid cellular processes of suspended immune cells at the molecular level, something that is not possible with current technology. It will enable the study cellular interactions with a great degree of control by enabling one to manipulate and increase the number of cellular interactions and follow them dynamically.

1014838 Huser该项目将开发一种独特的最先进的技术，以获得一种极其重要的细胞类别的功能和结构的快速三维图谱：免疫细胞。它将利用悬浮细胞在其最自然的环境（即自由悬浮，无基质存在）中的高空间和时间分辨率的快速光学成像。这种新仪器将允许人们以毫秒级的时间尺度测量和记录三维细胞的生理变化。该系统将导致多个科学学科的重大进步，即细胞成像，细胞生物学，免疫学，病毒学等，通过实时定量评估由于亚细胞水平环境变化而导致的细胞组织和重组。这种新型仪器将显著增强在分子水平上量化悬浮免疫细胞的快速细胞过程的能力，这是目前技术所无法实现的。它将使研究细胞相互作用具有很大程度的控制，使人们能够操纵和增加细胞相互作用的数量，并动态地跟踪它们。