SBIR Phase II: Evaluation of recombinant HED2 proteins as synergists for cellulosic biofuel production

这个小企业创新研究第二阶段项目将使用Expansin Domain-2蛋白的同系物来增强纤维素在大规模生物质消化成糖中的性能，用于生物燃料项目。 由于依赖石油燃料的经济、政治和环境影响，人们对使用生物燃料的兴趣越来越大。 纤维素生物质为生物燃料生产提供了易于获得的高容量原料。 目标的成功完成将优化未纯化的ZM 3，以在放大条件下用作商业化目的的纤维素酶。 与开发降低成本和提高从纤维素生物质生产乙醇的效率的战略相关的更广泛的影响包括减少石油使用带来的许多社会效益。 将来自可再生林业和农业原料的细胞壁生物质转化为生物燃料能够有效利用废料。 从环境的角度来看，广泛使用纤维素生物燃料作为替代可再生能源，可以大大减少温室气体排放，因为植物有能力从大气中去除二氧化碳。

This Small Business Innovation Research Phase II project will use a Homolog of Expansin Domain-2 protein to enhance cellulose performance in large-scale biomass digestion into sugars for biofuel projection. Due to the economics, politics and environmental impacts of reliance on petroleum-based fuel, there is growing interest in using biofuel. Cellulosic biomass provides a readily-available, high-volume feedstock for biofuel production. Successful completion of the objectives will optimize unpurified ZM3 for use as a cellulose synergist in scaled-up conditions for commercialization purposes. Broader impacts associated with developing strategies to decrease the costs and improve the efficiency of the production of ethanol from cellulosic biomass include numerous societal benefits resulting from reduction of petroleum use. Conversion of cell wall biomass from renewable forestry and agricultural feedstocks into biofuels enables the efficient use of waste materials. From an environmental standpoint, the widespread use of cellulose-based biofuel as an alternative renewable energy source could substantially reduce greenhouse gas emissions due to the ability of plants to remove CO2 from the atmosphere.