Collaborative Research: EAGER: Microfluidic assessment of chemotaxis towards different inorganic and organically complexed iron species by marine bacteria and phytoplankton

合作研究：EAGER：海洋细菌和浮游植物对不同无机和有机复合铁物种的趋化性的微流体评估

• 批准号: 0940494
• 负责人: Mark Wells
• 金额: $ 8.13万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0940494&HistoricalAwards=false
• 关键词: Collaborative; Research; EAGER; Microfluidic; assessment

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).There is increasing evidence that iron is important in regulating bulk phytoplankton production in large regions of the world oceans and also influences the speciation of phytoplankton. However, not all iron in the ocean is biologically 'available', making it difficult to obtain a quantitative understanding of these linkages. Currently available methods that rely on adding one or another chemical species of iron to natural seawater cultures are not ideal because they cannot control for thermo- or photochemical cycling of iron species within the cultures. Thus, the need exists for new analytical methods to show which forms of iron are preferentially utilized by different marine bacteria and phytoplankton species. A scientist from the University of California, Santa Cruz in collaboration with a researcher from Massachusetts Institute of Technology plan to determine whether a new microfluidics technique successfully used to study dissolved organic matter utilization in marine bacteria and phytoplankton can be adapted to determine the effect of chemical speciation on iron acquisition by these species. Specifically, the proponents plan to demonstrate that the microfluidics chemotaxis experiments can be carried out under trace metal clean conditions and show that marine bacteria and phytoplankton display chemotaxis towards a recognizable source of iron. As regards broader impacts, this research would provide the ocean sciences community with a new technique to document which forms of iron are preferentially utilized by different marine bacteria and phytoplankton species. One postdoc from Massachusetts Institute of Technology would be supported and trained as part of this EAGER award.

该奖项是根据2009年的《美国复苏与再投资法》（公法111-5）资助的。有越来越多的证据表明，铁在调节世界大海洋大区域的散装浮游植物生产方面很重要，也影响了浮游植物的物种形成。但是，并非所有海洋中的铁在生物学上都可以“可用”，因此很难获得对这些联系的定量理解。 目前依赖于在天然海水培养中添加一种或另一种化学物质的铁种类的可用方法并不理想，因为它们无法控制培养物中铁物种的热或光化学循环。因此，存在新的分析方法的需求，以表明不同海洋细菌和浮游植物物种优先利用哪种形式的铁。 加利福尼亚大学圣克鲁斯的一位科学家与马萨诸塞州技术研究所的一名研究人员合作，以确定是否成功地使用用于研究海洋细菌中溶解的有机物利用的新的微流体技术是否可以改编来确定这些物种对铁习惯的影响。 具体而言，支持者计划证明微流体趋化性实验可以在痕量金属清洁条件下进行，并表明海洋细菌和浮游植物对可识别的铁的来源表现出趋化性。 关于广泛的影响，这项研究将为海洋科学界提供一种新技术，以记录不同海洋细菌和浮游植物物种优先利用铁的形式。 来自马萨诸塞州理工学院的一位博士后将得到支持和培训，这是该渴望奖的一部分。