Environmental Genomics in an Antarctic polychaete

南极多毛类动物的环境基因组学

• 批准号: 0944557
• 负责人: Adam Marsh
• 金额: $ 40.69万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-15 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0944557&HistoricalAwards=false
• 关键词: Environmental; Genomics; Antarctic; polychaete

Genome-enabled biology provides a foundation for understanding the genetic basis of organism-environment interactions. . The research project links gene expression, genome methylation, and metabolic rates to assess the mechanisms of environmental adaptation (temperature) across multiple generations in a polar, and closely related temperate, polychaete. By comparing these two species, the research will assess how a polar environment shapes responses to environmental stress. This work will produce: 1) a database of full transcriptome (gene specific) profiling data for the polar polychaete cultured at two temperatures; 2) the contribution of genome methylation to the suppression of gene transcription activities; 3) the linkage between shifts in mRNA pools and total cellular activities (as ATP consumption via respiration); 4) an assessment of the inheritance of patterns of gene expression and metabolic activities across three generations; and 5) a simple demographic model of the polar polychaete population dynamics under normal and 'global-warming' temperature scenarios. Broader impacts include two outreach activities. The first is a mentoring program, where African-American undergraduate students spend 1.5 years working on a research project with a UD faculty member (2 summers plus their senior academic year). The second is a children's display activity at UD?s School of Marine Science "Coast Day".

基因组生物学为理解生物体与环境相互作用的遗传基础提供了基础。.该研究项目将基因表达，基因组甲基化和代谢率联系起来，以评估极地和密切相关的温带多毛类动物多代环境适应（温度）的机制。 通过比较这两个物种，该研究将评估极地环境如何塑造对环境压力的反应。 这项工作将产生：1）全转录组数据库在两种温度下培养的极地多毛类动物的（基因特异性）分析数据; 2）基因组甲基化对基因转录活性抑制的贡献; 3）mRNA库的变化与总细胞活性之间的联系（如通过呼吸的ATP消耗）; 4）评估三代间基因表达和代谢活动模式的遗传;和5）一个简单的人口模型的极地多毛类种群动态下正常和“全球变暖”的温度情景。更广泛的影响包括两项外联活动。 第一个是辅导计划，非洲裔美国本科生花1.5年的时间与UD教职员工一起从事研究项目（2个夏天加上他们的高年级学年）。第二个是UD的儿童展示活动。海洋科学学院的“海岸日”。