SBIR Phase I: Nanoparticle Sensor for Detection of E. coli in Real-time and Real-world Conditions

SBIR 第一阶段：用于在实时和真实条件下检测大肠杆菌的纳米颗粒传感器

• 批准号: 0945814
• 负责人: Melanie Tomczak
• 金额: $ 15万
• 依托单位: UES, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0945814&HistoricalAwards=false
• 关键词: SBIR; Phase; Nanoparticle; Sensor; Detection

This SBIR Phase I project will develop a passive sensor for real-time detection of E. coli without concentration steps. It is based on the change in conductivity which would occur when the bacterium is captured by peptidoglycans specific to that strain which have been immobilized on two closely-separated gold electrodes. A unique aspect of this work is the use of glycoligands as the sensing element. Advantages include instantaneous responses that are more robust and stable than antibodies and peptides; and are not subject to antigenic drift, greater sensitivity (as glycan-based ligands are smaller than antibodies, hence coverage on the sensor surface can be more dense), and linkage with virulence (as binding to glycan receptors in the host is required for virulence). A prototype of the sensor has already been developed for the Air Force, using peptides as the capture ligands, but it needs to be miniaturized.The broader/commercial impact of the proposed project will be in the monitoring of food and water supplies in real time. Presence of pathogens will be known in time to prevent poisoning from food and water. If successful, this device would be of great assistance to managers of beaches and similar recreational facilities, as well as drinking water treatment plant operators. There would be immediate results of tests to determine the presence of virulent E. coli. The commercial potential for this sensor extends beyond the water industry to include meat production and defense/security. The market for the water industry exceeds $1b annually. The aim is to market the sensor at ~$100 per test, which is competitive for critical health-based applications. The key benefit of the innovation lies in the fact that this solution would provide real-time detection, while the quickest detection system on the market today takes 4-7 hours to provide alerts.

该SBIR第一阶段项目将开发一种用于实时检测大肠杆菌的被动传感器。大肠杆菌中，没有浓缩步骤。 它是基于当细菌被固定在两个紧密分离的金电极上的对该菌株特异的肽聚糖捕获时会发生的电导率变化。 这项工作的一个独特方面是使用糖配体作为传感元件。 优点包括比抗体和肽更稳健和稳定的瞬时响应;并且不受抗原漂移的影响，更高的灵敏度（因为基于聚糖的配体比抗体小，因此传感器表面上的覆盖可以更密集），以及与毒力的联系（因为毒力需要与宿主中的聚糖受体结合）。 该传感器的原型已经为空军开发，使用肽作为捕获配体，但它需要小型化。拟议项目的更广泛/商业影响将是在真实的时间监测食品和水供应。 将及时了解病原体的存在，以防止食物和水中毒。 如果成功，该装置将对海滩和类似娱乐设施的管理人员以及饮用水处理厂的经营者有很大的帮助。 将有立即的测试结果，以确定是否存在有毒的E。杆菌 这种传感器的商业潜力超出了水行业，包括肉类生产和国防/安全。 水行业的市场每年超过10亿美元。 其目标是以每次测试约100美元的价格将传感器推向市场，这对于基于健康的关键应用来说具有竞争力。 这项创新的主要好处在于，该解决方案将提供实时检测，而目前市场上最快的检测系统需要4-7小时才能提供警报。