SBIR Phase I: Active Titanium Dioxide Nanoprobes as Biosensors for Detection and Diagnostics
SBIR 第一阶段:活性二氧化钛纳米探针作为检测和诊断的生物传感器
基本信息
- 批准号:0637888
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-01-01 至 2007-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This Small Business Innovation Research (SBIR) Phase I project will develop a biosensor to detect proteins or biomacromolecules (viruses, spores, etc.) in real time with increased specificity and decreased background compared with the current state-of-the-art. Currently, biosensors rely on solid-state, colorimetric or fluorescence assays that require expensive equipment for result evaluation. In this proposal, the technology is based on a biologically-active, photoexcitable nanoprobe that will trigger the activation of a reporter enzyme upon binding to the target of interest. In this Phase I project, two biosensors -- a surface sensor and a solution sensor -- will be developed based on the same bioconjugated nanoprobe (BNP) platform. These BNP sensor systems improve on the design of the sandwich immunoassay by utilizing multi-layered detection specificity towards the target of interest. The reporter signal is released only when multiple binding molecules are engaged concurrently, but is prevented from release if only one or none of the binding molecules are engaged. This design allows for differentiation between different isoforms or species of the target molecule as well as decreases background noise in the sensor. A sensor with these attributes would re-define the state-of-the-art for sensor and detection technology. In this Phase I project active bioconjugated nanoprobes will be developed into both surface and solution sensors. The surface sensor could be used as a local or remote detection device, with numeric results appearing on an LCD screen or sent back to a monitoring site, respectively. The solution sensor could be used instead of an ELISA assay thereby alleviating the need for a platereader, as the results could be interrogated with a spectrophotometer or simply as a "yes" or "no" visual result. Commercially, these biosensors would represent significant advances in detection of chemical and biological warfare and improvised explosive device agents, as their design increases the level of stringency, allows detection in real time as binding occurs, reports results without specialized detection equipment, and allows remote interrogation. The sensor design would differentiate between type of agents and decipher a mixture of agents reliably, eventually using a subdivided platform on a chip to achieve detection and differentiation of multiple targets simultaneously. The biosensor platform presented in this proposal represents a major advance in detection technology. This research should result in desirable commercial products in areas as diverse as the Department of Defense, Homeland Security, the biotechnology and pharmaceutical industries, and academia.
这个小企业创新研究(SBIR)第一阶段项目将开发一种生物传感器,用于检测蛋白质或生物大分子(病毒,孢子等)。与现有技术相比,具有增加的特异性和降低的背景的真实的时间。目前,生物传感器依赖于固态、比色或荧光测定,其需要昂贵的设备用于结果评估。在该提案中,该技术基于生物活性的光激发纳米探针,其将在与感兴趣的靶标结合时触发报告酶的激活。在这个第一阶段项目中,两个生物传感器-表面传感器和溶液传感器-将基于相同的生物共轭纳米探针(BNP)平台开发。这些BNP传感器系统通过利用针对感兴趣的靶标的多层检测特异性来改进夹心免疫测定的设计。仅当多个结合分子同时接合时才释放报告信号,但如果仅接合一个或没有接合结合分子,则防止释放报告信号。这种设计允许区分靶分子的不同同种型或种类,并降低传感器中的背景噪声。具有这些属性的传感器将重新定义传感器和检测技术的最新发展。在这个第一阶段项目中,活性生物共轭纳米探针将被开发成表面和溶液传感器。表面传感器可用作本地或远程检测设备,数字结果分别显示在LCD屏幕上或发送回监测现场。溶液传感器可用于代替ELISA测定,从而减轻对读板器的需要,因为结果可使用分光光度计询问或简单地作为“是”或“否”视觉结果。在商业上,这些生物传感器将代表在检测化学和生物战剂以及简易爆炸装置制剂方面的重大进展,因为它们的设计提高了严格程度,允许在结合发生时进行真实的检测,报告结果而无需专门的检测设备,并允许远程询问。传感器设计将区分不同类型的代理和可靠地破译代理的混合,最终使用芯片上的细分平台来同时实现多个目标的检测和区分。本提案中提出的生物传感器平台代表了检测技术的重大进步。这项研究应该会在国防部、国土安全部、生物技术和制药业以及学术界等不同领域产生理想的商业产品。
项目成果
期刊论文数量(0)
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Melanie Tomczak其他文献
Melanie Tomczak的其他文献
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