Fungal Genome and Transcriptomes in Phytopathogenesis

植物发病机制中的真菌基因组和转录组

• 批准号: 0947963
• 负责人: Michael Perlin
• 金额: $ 69.37万
• 依托单位: University of Louisville Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0947963&HistoricalAwards=false
• 关键词: Fungal; Genome; Transcriptomes; Phytopathogenesis

The Microbotryum violaceum fungal complex is an exciting system for examination of the ecology and evolution of host/pathogen interactions and can serve as a model for emerging infectious diseases in 'wild' non-agricultural environments where host heterogeneity provides an important contrast to heavily studied agricultural pathogens. Current genomics scale high-throughput technologies are invaluable for gaining insight into the complex systems and associated interactions that result in disease progression. This project will provide a genomics infrastructure necessary for the rational design of hypothesis driven inquiries into M. violaceum development and pathogenesis. A comprehensive and comparative view will be generated of the suites of genes that play significant roles in fungal development and successful infection. The specific objectives are to: (1) Sequence and assemble the complete genome of Microbotryum violaceum, which is estimated to be 25 Mb; (2) Annotate gene structure using computational methods; (3) Analyze gene transcription profiles late in infection; (4) Promptly release publicly all reads, assemblies, and annotation; and (5) Develop education, training and outreach programs. Broader impacts: Several research groups are prepared to use the genome sequence to analyze signaling leading to fungal development and disease production, host specificity, genes under positive selection, evolution of sex, and gene expression during infection. A website will be developed at the Broad Institute to disseminate the information on the genome of M. violaceum as well as allow comparison to other genomes, including Ustilago maydis, the causative agent of corn smut disease. These resources will be used by a community of scientists in the public and private sectors interested in basic fungal biology, host-parasite interactions, fungal evolution and the development of new methods for protecting agricultural crop plants from fungal diseases through a better understanding of the basic infection mechanism and disease process of obligate plant parasitic fungi. Furthermore, this project will provide educational opportunities for undergraduate students to participate in fungal genomics and bio-informatics research and broaden the participation in basic science by under-represented groups (specifically, African-Americans, women, Appalachians). An outreach mechanism will enhance the interest of such undergraduates in pursuing an advanced degree in the biological sciences.

的Microbotryum violaceum真菌复合体是一个令人兴奋的系统，用于检查宿主/病原体相互作用的生态和进化，并可以作为一个模型，为新兴的传染病在“野生”非农业环境中，宿主异质性提供了一个重要的对比，大量研究农业病原体。目前的基因组学规模高通量技术对于深入了解导致疾病进展的复杂系统和相关相互作用非常宝贵。该项目将提供一个必要的基因组学基础设施的合理设计的假设驱动调查到M。violaceum的发展和发病机制。一个全面的和比较的观点将产生的套件的基因，在真菌的发展和成功的感染中发挥重要作用。具体目标是：（1）测序和组装紫色微孢菌的完整基因组，估计为25 Mb;（2）使用计算方法注释基因结构;（3）分析感染后期的基因转录谱;（4）公开发布所有读数，组装和注释;以及（5）开发教育，培训和推广计划。 更广泛的影响：几个研究小组准备使用基因组序列来分析导致真菌发育和疾病产生的信号传导、宿主特异性、正选择下的基因、性别进化和感染期间的基因表达。布罗德研究所将建立一个网站，以传播关于M. violaceum的基因组，并允许与其他基因组进行比较，包括玉米黑粉菌，玉米黑穗病的病原体。这些资源将由公共和私营部门的科学家社区使用，这些科学家对基础真菌生物学、宿主-寄生虫相互作用、真菌进化以及通过更好地了解专性植物寄生真菌的基本感染机制和疾病过程来开发保护农作物免受真菌疾病侵害的新方法感兴趣。此外，该项目将为本科生提供参与真菌基因组学和生物信息学研究的教育机会，并扩大代表性不足的群体（特别是非洲裔美国人，妇女，阿巴拉契亚人）对基础科学的参与。 外展机制将提高这些本科生攻读生物科学高级学位的兴趣。