Genomweite Assoziationsstudie für Trockentoleranz in Arabidopsis thaliana Keimlingen

拟南芥幼苗耐旱性的全基因组关联研究

• 批准号: 195134946
• 负责人: Professor Dr. Arthur Korte, Ph.D.
• 金额: --
• 依托单位: Gregor-Mendel-Institut für Molekulare Pflanzenbiologie
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/195134946?language=en
• 关键词: Genomweite; Assoziationsstudie; Trockentoleranz; Arabidopsis; thaliana

Drought is a major abiotic stress affecting plants. On a global scale, water availability affects yield severely, a problem that will likely be exacerbated by climate change. It is thus of fundamental importance to generate crop plants that utilize water more efficiently and show greater resistance to drought. Although some progress in generating drought resistant plants has been made using conventional breeding or genetic engineering, our understanding of the molecular mechanisms underlying drought resistance in nature is still very poor. One approach to identify new genes or even networks of genes contributing to drought tolerance in natural accessions is genome-wide association studies (GWAS). Arabidopsis thaliana is the ideal tool for this kind of study, as large collections of genotyped accessions (natural inbred lines) already exist. The availability of inbred lines has the additional benefit that a genotyped line can be used for phenotypic analysis multiple times, either to achieve replication or to study genotype-environment interactions. Our objective here is to carry out GWAS for drought tolerance in seedlings in order to identify the major determinants of natural variation for this trait in A. thaliana. This analysis will lead to the identification of many putative new components of the signalling network that are relevant in natural environments. These components will be further validated by the combinatorial use of crossings and transgenic approaches. The end-result will be better understanding of the molecular network that contributes to drought tolerance in Arabidopsis thaliana and in plants in general.

干旱是影响植物的主要非生物胁迫。在全球范围内，水的供应严重影响产量，气候变化可能会加剧这一问题。因此，产生更有效地利用水并表现出更大的抗旱性的作物植物是至关重要的。虽然利用常规育种或基因工程在培育抗旱植物方面取得了一些进展，但我们对自然界抗旱性的分子机制的理解仍然很差。全基因组关联研究（GWAS）是鉴定天然种质中耐旱性新基因甚至基因网络的一种方法。拟南芥是这种研究的理想工具，因为已经存在大量基因型种质（天然近交系）。近交系的可用性具有额外的益处，即基因分型的系可以多次用于表型分析，以实现复制或研究基因型-环境相互作用。我们的目标是进行幼苗耐旱性的GWAS，以确定该性状在A. thaliana.这种分析将导致识别许多推定的新组件的信号网络，在自然环境中相关。这些组件将进一步验证杂交和转基因方法的组合使用。最终的结果将是更好地了解分子网络，有助于拟南芥和一般植物的耐旱性。