Genome-level resolution of species boundaries and phylogeny of the North American tiger salamander radiation

北美虎蝾螈辐射的物种边界和系统发育的基因组水平分辨率

• 批准号: 0949532
• 负责人: David Weisrock
• 金额: $ 45万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0949532&HistoricalAwards=false
• 关键词: Genome; level; resolution; species; boundaries

Tiger salamanders are used in a range of biological and biomedical research, with much emphasis placed on a single species, the Mexican Axolotl, due to ease of rearing and experimental manipulation. However, this species is just one representative of a large and diverse radiation of tiger salamanders. Collectively, this group can serve as a model system for research ranging from speciation to regeneration, but little is known about how many species actually exist and little is known about their natural history, evolution, development, and relationships. This research will leverage new molecular technologies and existing genetic data to provide a comprehensive assessment of tiger salamander species diversity across North America and place these species into a comparative framework to understand their evolution. Concurrently, there will be development of new statistical tools to identify genes that have been uniquely influenced by other factors, such as natural selection.This research forges a strong link between statistical and biological research programs, an increasingly important form of collaboration as Biology moves further into the realm of genomics. Support and training of human resources will be provided at the postdoctoral and graduate level and persons employed by this grant will be integrated into a modern and multidisciplinary research program. This work will result in the collection of one of the largest set of genetic data at the level of populations for a naturally distributed organism.

虎蝾螈被用于一系列生物和生物医学研究，由于易于饲养和实验操作，重点放在单一物种墨西哥蝾螈上。 然而，这一物种只是一个代表性的大型和多样化的辐射虎蝾螈。 总的来说，这个群体可以作为从物种形成到再生的研究的模型系统，但对实际存在多少物种知之甚少，对它们的自然历史，进化，发展和关系知之甚少。 这项研究将利用新的分子技术和现有的遗传数据，对整个北美的虎蝾螈物种多样性进行全面评估，并将这些物种置于一个比较框架中，以了解它们的进化。与此同时，将开发新的统计工具，以确定基因已独特地受到其他因素的影响，如自然选择。这项研究锻造了统计和生物学研究计划之间的紧密联系，随着生物学进一步进入基因组学领域，这是一种越来越重要的合作形式。 将在博士后和研究生一级提供人力资源的支持和培训，该补助金所雇用的人员将被纳入现代和多学科研究计划。 这项工作将导致在自然分布的生物种群一级收集最大的遗传数据集之一。