CAREER: Experiencing the 5th Dimension, cis/trans and Z/E Isomerizations in Biomolecules

职业:体验生物分子的第五维、顺式/反式和 Z/E 异构化

基本信息

  • 批准号:
    0952643
  • 负责人:
  • 金额:
    $ 89.88万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-03-01 至 2016-02-29
  • 项目状态:
    已结题

项目摘要

Proteins are the catalysts of life. For such a catalyst to work, substantial reorganization of the atomic structure of the catalyst itself is required. It would be ideal to follow the catalytic reaction directly from the beginning to the end by looking at all the structures on the atomic length scale and in real time. Time-resolved crystallography is designed to achieve this. This technique is able to record up to 10 billion X-ray structures per second. Time-resolved crystallography is a true kinetic method. It investigates an ensemble of molecules in four dimensions, 3 space dimensions and the time. In order to determine a comprehensive kinetic mechanism, an additional parameter, the temperature, will be varied. Crystallography becomes five-dimensional. This project implements five-dimensional crystallography and uses it to investigate two important biological reactions: the photo-cycle in photoactive yellow protein and the photo-optical switching reaction in a biliprotein. Amongst other things, these investigations will pave the way for example for a rational design of optical switches with new colors that can be used for many applications in biophysical research.Understanding biological catalysis is one of the most important fields in the life sciences. Time-resolved crystallography unifies kinetic with structure determination. With the advent of the world's strongest X-ray sources, the free electron lasers for hard X-rays, five-dimensional crystallography can be brought to utmost perfection in terms of speed and smallness of specimen. This will open new research opportunities. To attract young academics, the PI will introduce macromolecular crystallography to Milwaukee's high schools by training science teachers who work as mentees in the PI's lab. A broad educational program in biophysics at the UW-Milwaukee will attract bright undergraduate and graduate students to be included in ongoing projects. Numerous trips to the world's strongest X-ray sources will give plenty of hands-on opportunities. Results will be distributed through the PI's web page so that anyone can experience the fifth dimension in crystallography.
蛋白质是生命的催化剂。 为了使这种催化剂发挥作用,需要对催化剂本身的原子结构进行大量重组。通过实时观察原子长度尺度上的所有结构,直接从头到尾跟踪催化反应是理想的。时间分辨晶体学旨在实现这一目标。该技术每秒能够记录多达 100 亿个 X 射线结构。时间分辨晶体学是一种真正的动力学方法。它研究四个维度、三个空间维度和时间的分子集合。为了确定全面的动力学机制,将改变另一个参数,即温度。晶体学变成五维的。该项目实施了五维晶体学,并用它来研究两个重要的生物反应:光活性黄色蛋白中的光循环和胆蛋白中的光光转换反应。除此之外,这些研究将为合理设计具有新颜色的光开关铺平道路,这些光开关可用于生物物理研究的许多应用。了解生物催化是生命科学中最重要的领域之一。时间分辨晶体学将动力学与结构测定结合起来。随着世界上最强的X射线源、用于硬X射线的自由电子激光器的出现,五维晶体学在速度和样品的小型化方面可以达到极致。这将带来新的研究机会。为了吸引年轻学者,PI 将通过培训在 PI 实验室担任学员的科学教师,向密尔沃基的高中介绍大分子晶体学。威斯康星大学密尔沃基分校广泛的生物物理学教育计划将吸引聪明的本科生和研究生参与正在进行的项目。多次前往世界上最强的 X 射线源将提供大量实践机会。结果将通过 PI 的网页发布,以便任何人都可以体验晶体学的第五维。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Marius Schmidt其他文献

Sensitivity analysis of a source partitioning method for H2O and CO2 fluxes based on high frequency eddy covariance data: Findings from field data and large eddy simulations
基于高频涡度协方差数据的 H2O 和 CO2 通量源划分方法的灵敏度分析:现场数据和大涡模拟的结果
  • DOI:
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    6.2
  • 作者:
    A. Klosterhalfen;A. Moene;Marius Schmidt;Todd M. Scanlon;H. Vereecken;Alexander Graf
  • 通讯作者:
    Alexander Graf
Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources.
现代 X 射线源的时间分辨大分子晶体学。
  • DOI:
    10.1007/978-1-4939-7000-1_11
  • 发表时间:
    2017
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Marius Schmidt
  • 通讯作者:
    Marius Schmidt
New software for the singular value decomposition of time-resolved crystallographic data
用于时间分辨晶体数据奇异值分解的新软件
  • DOI:
  • 发表时间:
    2009
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Yi Zhao;Marius Schmidt
  • 通讯作者:
    Marius Schmidt
Time-Resolved Crystallography at X-ray Free Electron Lasers and Synchrotron Light Sources
X 射线自由电子激光器和同步加速器光源的时间分辨晶体学
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Marius Schmidt
  • 通讯作者:
    Marius Schmidt
Time-Resolved Serial Femtosecond Crystallography at the European X-ray Free Electron Laser
欧洲 X 射线自由电子激光器的时间分辨串行飞秒晶体学

Marius Schmidt的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Marius Schmidt', 18)}}的其他基金

RAPID: Structure, Function and Dynamics of SARS Coronavirus-2 Main Protease 3CLpro Determined with Mix-and-Inject Serial XFEL Crystallography
RAPID:使用混合注射串行 XFEL 晶体学测定 SARS 冠状病毒 2 主要蛋白酶 3CLpro 的结构、功能和动力学
  • 批准号:
    2030466
  • 财政年份:
    2020
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Standard Grant

相似海外基金

Making Every Community Asset Count: Improving Health and Reducing Inequalities for People Experiencing Homelessness
让每一项社区资产发挥作用:改善无家可归者的健康并减少不平等
  • 批准号:
    AH/Z505389/1
  • 财政年份:
    2024
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Research Grant
Test, treat, and connect on site using a dual rapid diagnostic point-of-care test for syphilis and HIV among people who are experiencing houselessness
使用梅毒和艾滋病毒双重快速诊断现场检测对无家可归者进行现场检测、治疗和联系
  • 批准号:
    489125
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Operating Grants
Improving pregnancy and reproductive health of people experiencing incarceration in Canada by mobilizing knowledge with community partners
通过与社区合作伙伴调动知识,改善加拿大被监禁者的怀孕和生殖健康
  • 批准号:
    476891
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
In my neighbourhood, in my city: A community engaged, multi-sectoral, equity driven approach to identifying opportunities for improving access and supports for racialized and LGBTQ2S+ women experiencing substance use challenges in the City of Toronto
在我的社区,在我的城市:采用社区参与、多部门、公平驱动的方法,为多伦多市面临药物使用挑战的种族和 LGBTQ2S 妇女寻找改善机会和支持的机会
  • 批准号:
    480869
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Miscellaneous Programs
Improving discharges of people experiencing homelessness from hospital: a quality improvement project embedded in a stepped wedge design
改善无家可归者出院情况:嵌入阶梯式楔形设计的质量改进项目
  • 批准号:
    487672
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Operating Grants
Supporting racialized youth experiencing technology-facilitated sexual violence and its impact on mental health: Engaging youth in co-designing policy and practice recommendations
支持遭受技术性暴力及其对心理健康影响的种族青年:让青年参与共同设计政策和实践建议
  • 批准号:
    499283
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Operating Grants
The impact of experiencing maternal violent injury on health outcomes in youth: an evaluation of health system impact and needs.
经历孕产妇暴力伤害对青少年健康结果的影响:对卫生系统影响和需求的评估。
  • 批准号:
    499274
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Operating Grants
Reading Pleasures: Experiencing the Early Modern Erotic
阅读乐趣:体验早期现代情色
  • 批准号:
    2888643
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Studentship
LEAP-HI: Compounding Risk Assessment and Mitigation Options for Building Infrastructure Experiencing Coastal Flooding-Related Saltwater Deterioration and Seismic Hazard
LEAP-HI:针对遭受沿海洪水相关的盐水恶化和地震灾害的基础设施建设的综合风险评估和缓解方案
  • 批准号:
    2245401
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
    Continuing Grant
A Longitudinal Qualitative Study of Fentanyl-Stimulant Polysubstance Use Among People Experiencing Homelessness (Administrative supplement)
无家可归者使用芬太尼兴奋剂多物质的纵向定性研究(行政补充)
  • 批准号:
    10841820
  • 财政年份:
    2023
  • 资助金额:
    $ 89.88万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了