Combining Imaging Mass Spectrometry and Capillary Electrophoresis to Decipher Chemical Signaling in the Nervous System in Response to Environmental Stress

结合成像质谱法和毛细管电泳来破译神经系统中响应环境压力的化学信号

• 批准号: 0957784
• 负责人: Lingjun Li
• 金额: $ 45万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-15 至 2014-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0957784&HistoricalAwards=false
• 关键词: Combining; Imaging; Mass; Spectrometry; Capillary

With this award from the Chemical Measurement and Imaging Program in the Chemistry Division, Professor Lingjun Li at the University of Wisconsin-Madison seeks to develop sensitive and versatile approaches to assessing the neurochemical response to environmental stress. Probes include mass spectrometric imaging and capillary electrophoresis, with novel tagging reagents and improved bioinformatics software. The tools will have broad applicability to analysis of biochemical and peptidergic signaling in nervous systems. Insights gained should lead to better understanding of the neuroendocrine regulatory mechanisms involved in adapting to environmental stress, a process that is highly conserved among all living organisms to maintain their survival in the face of both externally and internally generated stimuli. In simple terms, the long-term aim is better understanding of the detailed functioning of nervous systems. The tools will be promulgated rapidly to the neuroscience community through long-term and active collaborations with biologists in the field. Collaboration with industrial partners will also facilitate both improvement and promulgation of the CE-MS interface design. A large group of graduate and undergraduate students with diverse background (including many women scientists) is actively engaged. A partnership with the Madison Metropolitan School District (MMSD) provides opportunities to host and mentor high school students during summer research internships.

凭借化学部化学测量和成像计划的这一奖项，威斯康星大学麦迪逊分校的Lingjun Li教授寻求开发敏感和通用的方法来评估神经化学对环境压力的反应。 探针包括质谱成像和毛细管电泳，具有新型标记试剂和改进的生物信息学软件。该工具将具有广泛的适用性，在神经系统中的生化和肽能信号的分析。 获得的见解应导致更好地了解神经内分泌调节机制参与适应环境压力，这是一个高度保守的过程中，所有生物体，以维持其生存，在面对外部和内部产生的刺激。 简而言之，长期目标是更好地了解神经系统的详细功能。 这些工具将通过与该领域生物学家的长期和积极合作迅速向神经科学界公布。 与工业伙伴的合作也将促进CE-MS接口设计的改进和颁布。 一大批具有不同背景的研究生和本科生（包括许多女科学家）积极参与。 与麦迪逊大都会学区（MMSD）的合作伙伴关系提供了在夏季研究实习期间接待和指导高中生的机会。