CAREER: Development of Integrated MS Strategies for Probing Peptidergic Signaling

职业：开发用于探测肽能信号传导的综合 MS 策略

• 批准号: 0449991
• 负责人: Lingjun Li
• 金额: $ 54.6万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0449991&HistoricalAwards=false
• 关键词: CAREER; Development; Integrated; MS; Strategies

Professor Lingjun Li of the University of Wisconsin at Madison is supported by the Analytical and Surface Chemistry CAREER Program in the Chemistry Division to develop an array of novel mass spectrometry-based strategies, specifically, in situ peptide analysis directly from the stomatogastric nervous system and neurhemal organs of crustaceans, like the crab and the lobster, using ultra-high resolution matrix assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS). In addition, Professor Li will employ multidimensional separation schemes coupled to both MALDI-FTMS and electrospray ionization (ESI)- tandem mass spectrometry for de novo peptide sequencing, and use database homology searching to allow the discovery of a large number of novel neuropeptides. The goal is to increase understanding of neuropeptidergic signaling. Professor Li also plans to persue the development of in vivo monitoring of peptide differential display in response to varied physiological conditions. Collectively, the analytical tools developed through this research and the discovery of a large number of novel neuropeptides should have tremendous impact in both the analytical chemistry and the neuroscience research communities. The PI recruits entry-level college students, and involves regional high school teachers in research to allow for better communication of the cutting-edge research to the greater public community. A large number of peptides are responsible for signaling in the brain and the nervous system. Knowledge of the neuropeptide profile at the cellular and network level is critical for a better understanding of peptidergic signaling in neuronal circuitry. This research has a high potential impact on brain science.

麦迪逊的威斯康星州大学的Lingjun Li教授得到化学系分析和表面化学CAREER项目的支持，开发了一系列基于质谱的新策略，特别是直接从甲壳类动物（如螃蟹和龙虾）的胃神经系统和神经血管器官进行原位肽分析，使用超高分辨率基质辅助激光解吸/电离（MALDI）傅立叶变换质谱（FTMS）。 此外，李教授将采用多维分离方案结合MALDI-FTMS和电喷雾离子化（ESI）-串联质谱进行从头肽测序，并使用数据库同源性搜索来发现大量新的神经肽。 目的是增加对神经肽能信号的理解。 李教授亦计划继续发展在不同生理条件下的肽差异显示的体内监测。 总的来说，通过这项研究开发的分析工具和大量新神经肽的发现应该在分析化学和神经科学研究界产生巨大的影响。 PI招募入门级大学生，并让地区高中教师参与研究，以便更好地将前沿研究与更广泛的公众社区进行沟通。 大量的肽负责大脑和神经系统中的信号传导。在细胞和网络水平的神经肽谱的知识是至关重要的，更好地了解肽能信号在神经元回路。 这项研究对脑科学有很大的潜在影响。