MRI-R2: Acquisition of an LCMS System to Enhance Undergraduate Research that Interfaces Chemistry and Biology

该奖项是根据2009年美国复苏和再投资法案（公法111 - 5）资助的。凭借这项来自主要研究和仪器（MRI）计划的奖项，Kevin P. Minbiole和同事丹尼尔M。作者：唐尼作者：Gina M.作者声明：MacDonald and Debra L.詹姆斯麦迪逊大学的莫勒将获得一个液相色谱接口质谱仪（LC-MS）。该仪器将用于本科生的研究活动，并在先进的本科化学类。待调查的项目包括青蛙和蝾螈皮肤细菌的抗真菌代谢物的特性，确定雪兰多河流域的内分泌受体化合物，测量血液中的神经递质代谢物，以及研究DNA和染色质之间的相互作用。 与Gallaudet大学和玛丽鲍德温学院的合作将使聋人和重听学生以及大量妇女参与重要的研究工作。 LC-MS结合了液相色谱的物理分离能力和质谱的质量分析能力。液相色谱仪把混合物分离成分子组分。 这些成分流入质谱仪，在那里测量它们的质量及其碎片的质量。 这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。 该奖项提供的仪器将为教师和本科生提供在化学和生物学界面进行重大合作研究的机会。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). With this award from the Major Research and Instrumentation (MRI) program, Kevin P. Minbiole and colleagues Daniel M. Downey, Reid N. Harris, Gina M. MacDonald and Debra L. Mohler of James Madison University will acquire a liquid chromatograph interfaced mass spectrometer (LC-MS). The instrument will be used in undergraduate research activities and in advanced undergraduate chemistry classes. Projects to be investigated include characterization of anti-fungal metabolites from the skin bacteria of frogs and salamanders, determination of endocrine receptor compounds in the Shenandoah River watershed, measurement of neurotransmitter metabolites in blood, and studies of the interactions between DNA and chromatin. Collaborations with Gallaudet University and Mary Baldwin College will allow deaf and hard of hearing students as well as substantial numbers of women to participate in important research endeavors. A LC-MS combines the physical separation capability of liquid chromatography with the mass analysis ability of mass spectrometry. The liquid chromatograph separates a mixture into its molecular components. These components flow into a mass spectrometer where their masses, and those of their fragments, are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. The instrumentation provided by this award will provide faculty and undergraduate students the opportunity to pursue significant collaborative investigations at the interface of chemistry and biology.