MRI-R2: Acquisition of an LCMS System to Enhance Undergraduate Research that Interfaces Chemistry and Biology

MRI-R2：收购 LCMS 系统以加强化学和生物学交叉的本科研究

• 批准号: 0958973
• 负责人: Christine Hughey
• 金额: $ 29.98万
• 依托单位: James Madison University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0958973&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; LCMS; System

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). With this award from the Major Research and Instrumentation (MRI) program, Kevin P. Minbiole and colleagues Daniel M. Downey, Reid N. Harris, Gina M. MacDonald and Debra L. Mohler of James Madison University will acquire a liquid chromatograph interfaced mass spectrometer (LC-MS). The instrument will be used in undergraduate research activities and in advanced undergraduate chemistry classes. Projects to be investigated include characterization of anti-fungal metabolites from the skin bacteria of frogs and salamanders, determination of endocrine receptor compounds in the Shenandoah River watershed, measurement of neurotransmitter metabolites in blood, and studies of the interactions between DNA and chromatin. Collaborations with Gallaudet University and Mary Baldwin College will allow deaf and hard of hearing students as well as substantial numbers of women to participate in important research endeavors. A LC-MS combines the physical separation capability of liquid chromatography with the mass analysis ability of mass spectrometry. The liquid chromatograph separates a mixture into its molecular components. These components flow into a mass spectrometer where their masses, and those of their fragments, are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. The instrumentation provided by this award will provide faculty and undergraduate students the opportunity to pursue significant collaborative investigations at the interface of chemistry and biology.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。詹姆斯·麦迪逊大学的Kevin P. Minbiole及其同事Daniel M. Downey、Reid N. Harris、Gina M. MacDonald和Debra L. Mohler获得了来自主要研究和仪器（MRI）项目的这项奖励，他们将获得一台液相色谱界面质谱仪（LC-MS）。该仪器将用于本科研究活动和本科高级化学课程。研究项目包括青蛙和蝾螈皮肤细菌抗真菌代谢物的表征，谢南多厄河流域内分泌受体化合物的测定，血液中神经递质代谢物的测量，以及DNA和染色质之间相互作用的研究。与加劳德特大学和玛丽鲍德温学院的合作将使失聪和重听学生以及大量女性参与重要的研究工作。LC-MS结合了液相色谱的物理分离能力和质谱的质量分析能力。液相色谱仪将混合物分离成分子组分。这些成分流入质谱仪，在那里它们的质量和碎片的质量被测量出来。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。该奖项提供的仪器将为教师和本科生提供机会，在化学和生物学的界面上进行重要的合作研究。