MRI-R2: Acquisition of a Liquid Handling Robot and a High-throughput Real-time PCR Thermocycler for Genetics and Genomics Research at UND

MRI-R2：UND 采购用于遗传学和基因组学研究的液体处理机器人和高通量实时 PCR 热循环仪

• 批准号: 0959369
• 负责人: Turk Rhen
• 金额: $ 39.77万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959369&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; Liquid; Handling

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the acquisition of an integrated set of instruments to enable medium to high-throughput analysis of DNA, RNA, and protein at the University of North Dakota (UND). A high capacity PCR unit, tissue homogenizer, and associated liquid handling robot greatly enhance the UND infrastructure to support biological research. The instrumentation enables cutting-edge research training and classroom education in engineering and life sciences at the interface of disciplines, i.e., ecological genomics, systems biology, and the human-robot interface. The research is unified by an emphasis on the genetic, epigenetic, and genomic bases of biological responses to environmental stressors, including abiotic factors like temperature, atmospheric gases, humidity, and heavy metals and biotic factors like herbivores and nutrition. The influence of high-throughput technologies reaches beyond pure research to provide significant new educational opportunities for undergraduate and graduate students. Such training opportunities are especially important for students who are demographically underrepresented in the life sciences and engineering. The nature of modern research is epitomized by the newly acquired instruments, stemming from interdisciplinary interactions among engineers, computer scientists, physicists, chemists, and biologists. The instrumentation allows students to gain valuable experiences, and may allow for the attraction or formation of new companies and higher paying jobs to the area, which in turn helps retain the state's talent and brainpower. Results from the studies enabled by the new equipment will be disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。这项主要研究仪器-回收和再投资（MRI-R2）奖励资助北达科他州大学（UND）购买一套集成仪器，以实现中到高通量的DNA， RNA和蛋白质分析。高容量PCR装置，组织均质机和相关的液体处理机器人极大地增强了UND的基础设施，以支持生物学研究。该仪器使工程和生命科学的前沿研究培训和课堂教育在学科的界面，即生态基因组学，系统生物学和人机界面。该研究统一于强调对环境压力源的生物反应的遗传、表观遗传和基因组基础，包括非生物因素，如温度、大气气体、湿度和重金属，以及生物因素，如食草动物和营养。高通量技术的影响超越了纯粹的研究，为本科生和研究生提供了重要的新的教育机会。这样的培训机会对于那些在生命科学和工程专业中人数不足的学生来说尤为重要。现代研究的本质是由工程师、计算机科学家、物理学家、化学家和生物学家之间跨学科的互动所产生的新获得的仪器所体现的。仪器仪表使学生获得宝贵的经验，并可能允许吸引或形成新的公司和高薪工作到该地区，这反过来有助于保留国家的人才和人才。由新设备促成的研究结果将通过学生和教员在区域和国家会议上的发言以及通过在同行评议的期刊上发表来传播。

项目成果

Evolutionary Turnover in Wnt Gene Expression but Conservation of Wnt Signaling during Ovary Determination in a TSD Reptile

• DOI: 10.1159/000516973
• 发表时间: 2021-07
• 期刊: Sexual Development
• 影响因子: 2.3
• 作者: T. Rhen;Zachary Even;Alaina Brenner;Alexandra Lodewyk;Debojyoti Das;Sunil Kumar Singh;R. Simmons