Genomic and Genetic Analysis of Temperature-dependent Gender Determination

温度依赖性性别决定的基因组和遗传分析

• 批准号: 0923300
• 负责人: Turk Rhen
• 金额: $ 46.78万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923300&HistoricalAwards=false
• 关键词: Genomic; Genetic; Analysis; Temperature; dependent

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). Temperature-dependent sex determination (TSD) was first reported 40 years ago in an African lizard. It has since been shown that temperature determines sex in some fish and amphibians, several lizards, numerous turtles, and all crocodilians. Nevertheless, the mechanism underlying TSD is not known in any species. The goal of the proposed research is to dissect the molecular basis of TSD in the common snapping turtle, Chelydra serpentina. This will be accomplished by (1) sequencing all genes expressed in gonads from embryos incubated at male- and female-producing temperatures, (2) breeding adult turtles to study genetic influences on sex determination in their offspring, and (3) genetic analysis of gene expression profiles in a subset of offspring from the breeding study. This information will be integrated to identify the temperature-sensitive gene (or genes) responsible for TSD and to reveal the gene networks for testis and ovary development, which is basically the same in all vertebrates.These experiments promise to get us closer to understanding the mechanism underlying TSD than has the previous 40 years of research. This work may provide new insight into the effects of climate change on endangered TSD species like sea turtles and a deeper understanding of sex determination in all vertebrates, including humans. Sequences for thousands of turtle genes could also be used for studies of other biological processes in turtles. Finally, this project will have a broader impact on education. Aspects of this research will be incorporated into undergraduate biology classes at UND. This represents a major advance in experiential learning of molecular biology for majors and non-majors. In addition, women, minorities, and North Dakota residents will be recruited into the PI?s laboratory, which will help increase the diversity of the nation?s scientists.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。温度依赖性性别决定（TSD）在40年前首次在非洲蜥蜴中报道。从那以后，温度决定了一些鱼类和两栖动物、几种蜥蜴、许多海龟和所有鳄鱼的性别。然而，TSD的潜在机制在任何物种中都是未知的。这项研究的目的是剖析普通鳄龟（Chelydra serpentina）TSD的分子基础。这将通过（1）对在雄性和雌性产生温度下孵育的胚胎的性腺中表达的所有基因进行测序，（2）繁殖成年海龟以研究遗传对后代性别决定的影响，以及（3）对繁殖研究中的一部分后代的基因表达谱进行遗传分析。这些信息将被整合，以确定负责TSD的温度敏感基因（或基因），并揭示睾丸和卵巢发育的基因网络，这在所有脊椎动物中基本上是相同的。这些实验有望使我们更接近了解TSD的机制比过去40年的研究。这项工作可能会为气候变化对海龟等濒危TSD物种的影响提供新的见解，并加深对包括人类在内的所有脊椎动物性别决定的理解。成千上万的海龟基因序列也可以用于研究海龟的其他生物过程。最后，该项目将对教育产生更广泛的影响。这项研究的各个方面将纳入UND的本科生物学课程。这代表了专业和非专业分子生物学体验式学习的重大进步。此外，妇女，少数民族和北达科他州居民将被招募到PI？的实验室，这将有助于增加国家的多样性？的科学家。

项目成果

Evolutionary Turnover in Wnt Gene Expression but Conservation of Wnt Signaling during Ovary Determination in a TSD Reptile

• DOI: 10.1159/000516973
• 发表时间: 2021-07
• 期刊: Sexual Development
• 影响因子: 2.3
• 作者: T. Rhen;Zachary Even;Alaina Brenner;Alexandra Lodewyk;Debojyoti Das;Sunil Kumar Singh;R. Simmons

Embryonic Temperature Programs Phenotype in Reptiles

胚胎温度决定爬行动物的表型

• DOI: 10.3389/fphys.2020.00035
• 发表时间: 2020
• 期刊: Frontiers in Physiology
• 影响因子: 4
• 作者: Singh, Sunil Kumar;Das, Debojyoti;Rhen, Turk
• 通讯作者: Rhen, Turk