MRI-R2: Acquisition of a high-intensity X-ray diffractometer for macromolecular crystallography

MRI-R2：购买用于大分子晶体学的高强度 X 射线衍射仪

• 批准号: 0959442
• 负责人: Martin St. Maurice
• 金额: $ 60.11万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959442&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; intensity; ray

Abstract This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the acquisition of an X-ray diffractometer to support basic research programs through the departments of Biological Sciences and Chemistry at Marquette University. The instrument also has broad inter-institutional use, supporting the basic research programs of additional faculty members at regional institutions. The diffractometer supports and enhances research programs that address structural aspects of biological macromolecules in a wide variety of systems. These programs include research on the structure and function of metabolic enzymes, protein-protein interactions contributing to essential cellular complexes, structural mechanisms of gene transcription and regulation, and the structure and function of proteins with antibacterial or antifungal activity. Structural biology is integrally tied to an undergraduate education in biology, biochemistry, and chemistry. The X-ray diffractometer at Marquette will be used to expose both graduate and undergraduate students to the increasingly accessible technique of macromolecular crystallography. Projects that involve the crystallization of proteins and DNA are well suited to undergraduate researchers who are gaining their first experience in the laboratory. Undergraduate students conducting independent research at Marquette learn crystallization techniques and gain first-hand experience with the X-ray diffractometer. Demonstrations are also performed for high school students in the Milwaukee School of Engineering's "Students Modeling a Research Topic" (SMART) program and in Marquette's Equal Opportunities Program. The addition of the X-ray diffractometer for macromolecular crystallography complements Marquette's infrastructure in NMR and small molecule X-ray crystallography, and enhances Marquette's ability to perform cutting edge research while maintaining our dedication to providing students with a diverse spectrum of research activities and opportunities. Results from the studies enabled by the new equipment will be disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.

本奖项由2009年美国复苏与再投资法案（公法111-5）资助。该主要研究仪器-回收和再投资（MRI-R2）奖资助购买x射线衍射仪，以支持马凯特大学生物科学和化学系的基础研究项目。该仪器还具有广泛的机构间使用，支持区域机构额外教员的基础研究项目。衍射仪支持和加强研究方案，解决结构方面的生物大分子在各种各样的系统。这些项目包括研究代谢酶的结构和功能，蛋白质-蛋白质相互作用对基本细胞复合物的贡献，基因转录和调控的结构机制，以及具有抗菌或抗真菌活性的蛋白质的结构和功能。结构生物学与生物学、生物化学和化学的本科教育密不可分。马奎特大学的x射线衍射仪将用于向研究生和本科生展示日益普及的大分子晶体学技术。涉及蛋白质和DNA结晶的项目非常适合在实验室中获得第一次经验的本科生研究人员。在马奎特大学进行独立研究的本科生学习结晶技术，并获得x射线衍射仪的第一手经验。在密尔沃基工程学院的“学生建模研究课题”（SMART）计划和马奎特大学的平等机会计划中，也为高中生进行了示范。大分子晶体学的x射线衍射仪的增加补充了马奎特大学在核磁共振和小分子x射线晶体学方面的基础设施，并增强了马奎特大学进行尖端研究的能力，同时保持我们致力于为学生提供多样化的研究活动和机会。由新设备促成的研究结果将通过学生和教员在区域和国家会议上的发言以及通过在同行评议的期刊上发表来传播。