MRI: Acquisition of a small volume isothermal titration calorimeter

马奎特大学获得了一个小体积等温滴定量热计（ITC），用于支持生物科学和化学部门的研究和教育。ITC允许在单个实验中同时测量多个结合参数，允许对溶液中生物分子相互作用进行定量热力学分析。高灵敏度、小体积仪器特别适用于样品制备耗时且昂贵的生命科学研究。该奖项将加强旨在(a)描述蛋白质和小分子之间结合相互作用的研究工作，这将揭示代谢和细胞信号传导酶的新机制细节；(b)表征蛋白质：蛋白质、蛋白质：核苷酸和蛋白质：脂质在参与维持细胞稳态的调节系统中的相互作用；(c)测量生物分子结合事件的详细热力学特征，以补充和增强现有的光谱方法。该奖项将为马奎特大学和康科迪亚大学的生物化学和生物物理研究项目提供新的仪器，并将扩大密尔沃基研究社区的生物物理基础设施。ITC仪器将通过将其纳入现有的生物化学和生物物理学高级实验课程来支持马奎特大学的教育活动。参加生物和化学本科研究项目的学生也可以使用。ITC仪器易于操作，使本科生获得获得和解释热力学数据的第一手经验，这些数据是现代生物化学和生物物理学的核心。

An award is made to Marquette University for the acquisition of a small volume isothermal titration calorimeter (ITC) for the purpose of supporting research and education in the departments of Biological Sciences and Chemistry. ITC allows simultaneous measurements of multiple binding parameters in a single experiment, allowing quantitative thermodynamic analyses of biomolecular interactions in solution. A highly sensitive, small-volume instrument is particularly amenable to research in the life sciences, where sample preparation is time-consuming and expensive. This award will enhance research efforts aimed at (a) characterizing binding interactions between proteins and small molecules that will reveal new mechanistic details for metabolic and cell signaling enzymes; (b) characterizing protein:protein, protein:nucleotide and protein:lipid interactions in regulatory systems involved in maintaining cellular homeostasis; (c) measuring detailed thermodynamic profiles of biomolecular binding events in order to complement and enhance existing spectroscopic methods. This award will provide new instrumentation to support biochemical and biophysical research programs at both Marquette University and Concordia University, and will expand the biophysical infrastructure available to the broader Milwaukee research community. The ITC instrument will support educational initiatives at Marquette University through its incorporation into existing advanced laboratory courses in biochemistry and biophysics. It will also be accessible to students participating in undergraduate research programs in both biology and chemistry. The ITC instrument is easy to operate, allowing undergraduate students to gain first-hand experience in obtaining and interpreting thermodynamic data that are central to modern biochemistry and biophysics.