MRI: Acquisition of a small volume isothermal titration calorimeter

MRI：获取小体积等温滴定量热计

• 批准号: 1337676
• 负责人: Martin St. Maurice
• 金额: $ 10.25万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337676&HistoricalAwards=false
• 关键词: MRI; Acquisition; small; volume; isothermal

An award is made to Marquette University for the acquisition of a small volume isothermal titration calorimeter (ITC) for the purpose of supporting research and education in the departments of Biological Sciences and Chemistry. ITC allows simultaneous measurements of multiple binding parameters in a single experiment, allowing quantitative thermodynamic analyses of biomolecular interactions in solution. A highly sensitive, small-volume instrument is particularly amenable to research in the life sciences, where sample preparation is time-consuming and expensive. This award will enhance research efforts aimed at (a) characterizing binding interactions between proteins and small molecules that will reveal new mechanistic details for metabolic and cell signaling enzymes; (b) characterizing protein:protein, protein:nucleotide and protein:lipid interactions in regulatory systems involved in maintaining cellular homeostasis; (c) measuring detailed thermodynamic profiles of biomolecular binding events in order to complement and enhance existing spectroscopic methods. This award will provide new instrumentation to support biochemical and biophysical research programs at both Marquette University and Concordia University, and will expand the biophysical infrastructure available to the broader Milwaukee research community. The ITC instrument will support educational initiatives at Marquette University through its incorporation into existing advanced laboratory courses in biochemistry and biophysics. It will also be accessible to students participating in undergraduate research programs in both biology and chemistry. The ITC instrument is easy to operate, allowing undergraduate students to gain first-hand experience in obtaining and interpreting thermodynamic data that are central to modern biochemistry and biophysics.

为支持生物科学系和化学系的研究和教学，马奎特大学购买了一台小容量等温滴定量热计(ITC)。ITC允许在单个实验中同时测量多个结合参数，从而可以对溶液中的生物分子相互作用进行定量热力学分析。高灵敏度、小体积的仪器特别适合在生命科学领域进行研究，因为在生命科学领域，样品制备既耗时又昂贵。该奖项将加强旨在以下方面的研究工作：(A)表征蛋白质和小分子之间的结合作用，以揭示代谢和细胞信号酶的新的机制细节；(B)表征蛋白质：蛋白质、蛋白质：核苷酸和蛋白质：参与维持细胞动态平衡的调节系统中的脂类相互作用；(C)测量生物分子结合事件的详细热力学轮廓，以补充和改进现有的光谱方法。该奖项将提供新的仪器来支持马奎特大学和康科迪亚大学的生化和生物物理研究项目，并将扩大密尔沃基研究界可用的生物物理基础设施。国际贸易中心的仪器将通过将其纳入现有的生物化学和生物物理学高级实验室课程，支持马奎特大学的教育举措。它也将对参加生物和化学本科研究项目的学生开放。ITC仪器操作简单，使本科生在获得和解释热力学数据方面获得第一手经验，而热力学数据是现代生物化学和生物物理学的核心。