MRI: Acquisition of a small volume isothermal titration calorimeter

MRI：获取小体积等温滴定量热计

• 批准号: 1337676
• 负责人: Martin St. Maurice
• 金额: $ 10.25万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337676&HistoricalAwards=false
• 关键词: MRI; Acquisition; small; volume; isothermal

An award is made to Marquette University for the acquisition of a small volume isothermal titration calorimeter (ITC) for the purpose of supporting research and education in the departments of Biological Sciences and Chemistry. ITC allows simultaneous measurements of multiple binding parameters in a single experiment, allowing quantitative thermodynamic analyses of biomolecular interactions in solution. A highly sensitive, small-volume instrument is particularly amenable to research in the life sciences, where sample preparation is time-consuming and expensive. This award will enhance research efforts aimed at (a) characterizing binding interactions between proteins and small molecules that will reveal new mechanistic details for metabolic and cell signaling enzymes; (b) characterizing protein:protein, protein:nucleotide and protein:lipid interactions in regulatory systems involved in maintaining cellular homeostasis; (c) measuring detailed thermodynamic profiles of biomolecular binding events in order to complement and enhance existing spectroscopic methods. This award will provide new instrumentation to support biochemical and biophysical research programs at both Marquette University and Concordia University, and will expand the biophysical infrastructure available to the broader Milwaukee research community. The ITC instrument will support educational initiatives at Marquette University through its incorporation into existing advanced laboratory courses in biochemistry and biophysics. It will also be accessible to students participating in undergraduate research programs in both biology and chemistry. The ITC instrument is easy to operate, allowing undergraduate students to gain first-hand experience in obtaining and interpreting thermodynamic data that are central to modern biochemistry and biophysics.

马奎特大学获得了一个小体积等温滴定量热仪（ITC），用于支持生物科学和化学系的研究和教育。ITC允许在一个单一的实验中同时测量多个结合参数，允许在溶液中的生物分子相互作用的定量热力学分析。高灵敏度、小体积的仪器特别适合生命科学研究，因为样品制备耗时且昂贵。该奖项将加强研究工作，旨在（a）表征蛋白质和小分子之间的结合相互作用，这将揭示代谢和细胞信号传导酶的新机制细节;（B）表征蛋白质：蛋白质，蛋白质：核苷酸和蛋白质：脂质在维持细胞稳态的调控系统中的相互作用;（c）测量生物分子结合事件的详细热力学概况，以补充和增强现有的光谱方法。该奖项将提供新的仪器，以支持马奎特大学和康考迪亚大学的生物化学和生物物理研究计划，并将扩大生物物理基础设施提供给更广泛的密尔沃基研究界。ITC仪器将通过将其纳入现有的生物化学和生物物理学高级实验室课程，支持马奎特大学的教育举措。它也将提供给参加生物学和化学本科研究项目的学生。ITC仪器易于操作，使本科生能够获得获得和解释热力学数据的第一手经验，这些数据对现代生物化学和生物物理学至关重要。