MRI-R2 Consortium: Development of an 8kx8k pixel direct detection CMOS camera with single electron counting for cryoEM

MRI-R2 联盟：开发用于冷冻电镜的具有单电子计数功能的 8kx8k 像素直接检测 CMOS 相机

• 批准号: 0960271
• 负责人: David Agard
• 金额: $ 197.68万
• 依托单位: University of California-San Francisco
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0960271&HistoricalAwards=false
• 关键词: MRI; R2; Consortium; Development; 8kx8k

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the development of an 8kx8k pixel direct detection CMOS camera with single electron quantized detection for high-resolution single particle cryo electron microscopy (cryoEM) at the University of California-San Francisco (UCSF). The new camera system will enable high frame readout rates, with extreme sensitivity and high resolution. Because it uses so little material and does not require crystals, single particle cryoEM has become an indispensable tool for studying the three-dimensional (3D) structures of complex biological assemblies, providing critical information not obtainable by more traditional methods such as x-ray crystallography or NMR spectroscopy. The camera development project will be coordinated through collaborations among UCSF, the Lawrence Berkeley National Laboratory (LBNL), and Gatan, Inc., a company specializing in EM cameras and peripherals. The camera will benefit a large number of research projects and improve the training prospects for 15-25 students and postdoctoral fellows in advanced cryoEM technology. UCSF PIs are also active participants in summer research programs and have been hosting undergraduates in their labs to provide research opportunities with advanced technologies. Performance and availability of the camera will be widely disseminated through a web site, in publications, and through presentations at local and international meetings. Commercialization of the new technology may lead to even wider dissemination of similar devices.

该奖项是根据2009年《美国复苏与再投资法案》(Public Law 111-5)资助的。这一重大研究仪器-复苏与再投资(MRI-R2)奖项资助加州大学旧金山分校(UCSF)为高分辨率单粒子低温电子显微镜(CryoEM)开发具有单电子量化检测功能的8kx8k像素直接检测CMOS相机。新的摄像系统将实现高帧读取率，具有极高的灵敏度和高分辨率。由于它使用的材料很少，而且不需要晶体，单粒子低温电子显微镜已经成为研究复杂生物组件的三维(3D)结构的不可或缺的工具，提供了X射线结晶学或核磁共振光谱等更传统方法无法获得的关键信息。相机开发项目将通过加州大学旧金山分校、劳伦斯伯克利国家实验室(LBNL)和专门从事EM相机和外围设备的Gatan公司之间的合作进行协调。该相机将使大量研究项目受益，并改善15-25名学生和博士后在先进低温EM技术方面的培训前景。加州大学旧金山分校的PI也是暑期研究项目的积极参与者，并一直在实验室接待本科生，为他们提供先进技术的研究机会。摄像机的性能和供应情况将通过网站、出版物以及在当地和国际会议上的介绍广泛传播。这项新技术的商业化可能会导致类似设备的更广泛传播。