MRI-R2 Consortium: Acquisition of multiple Environmental Sample Processors (ESPs) and supporting mooring and communications hardware

“这项奖励是根据2009年美国复苏和再投资法案（公法111-5）资助的。”π吗?我们请求资金购买5台环境样本处理器（esp）和支持系泊和通信硬件，以进一步开展研究活动并促进合作。ESP吗?S将一起部署在阵列配置中，以获取各种微生物及其代谢物的实时数据。多年来，这一直是一个遥远的梦想，但随着环境样本处理器（ESP）的发展和商业化，这个梦想现在接近现实，ESP是本提案的核心。ESP可在地下作业数月。它收集和处理水样，识别和列举有害藻华（HAB）物种、病原体和其他微生物以及特定代谢物（如藻类毒素）的浓度，并将数据传回岸上。为了培养更大的灵活性，ESP设计了一个微流控块，允许将不同类型的分析模块（例如定量PCR）放置在水处理核心的下游。科学和社会感兴趣的各种各样的生物和化学物质可以在时间尺度上进行分析，这是不可能的，所有这些都以自动化的方式进行。更广泛的影响潜在的更广泛的影响包括：许多本科生、研究生、博士后、技术人员和通过各种项目与esp合作的高级教师的教育和推广效益；WHOI和Biosecurity， Inc.为本科生（包括少数民族学生）提供的各种暑期实习项目；蒙特利湾水族馆将ESP纳入了一个每天两次的项目中，每年有近30万人参加；来自11个不同州的志愿浮游植物研究人员将在各种培训研讨会上接触ESP；科学地说，在海洋中放置更多的ESP，并最终安装到移动平台上，将使我们有可能像现在的CTD测量一样，提供信息并推动自适应采样；测试和建立ESP技术不仅将进一步推进所提出的研究，而且有助于制造和销售先进科学仪器的商业利益。随着这项技术得到证实，其他研究小组将寻求将其纳入其中，对相同或类似仪器的需求将会增长；该提案支持的许多启用/增强的项目（研究和教育）本质上是多研究者，甚至是多机构的，并将进一步增加相关研究小组之间的网络和伙伴关系。

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."The PI?s request funds to acquire five Environmental Sample Processors (ESPs) and supporting mooring and communications hardware to further their research activities and foster collaborations. The ESP?s will be deployed together in an array configuration to obtain real-time data on a wide range of microorganisms and their metabolites. For many years, this has been a distant dream, but that dream is now close to reality with the development and commercialization of the Environmental Sample Processor (ESP) that is the centerpiece of this proposal. The ESP can be deployed subsurface for months at a time. It collects and processes water samples, identifies and enumerates harmful algal bloom (HAB) species, pathogens, and other microorganisms as well as the concentration of specific metabolites such as algal toxins, and relays the data to shore. To foster greater flexibility, the ESP has been designed with a microfluidic block which allows analytical modules of different types (e.g., quantitative PCR) to be placed downstream of the water processing core. A wide variety of organisms and chemicals of interest to science and society can be analyzed over time scales that are not otherwise possible, all in automated fashion. Broader ImpactsThe potential broader impacts include: educational and outreach benefits from numerous undergraduates, graduate students, post docs, technical staff, and senior faculty working with the ESPs through various projects; various summer internship programs provided by WHOI and Biosecurity, Inc. for undergraduates including minority students; Monterey Bay Aquarium has included the ESP in a program that runs twice a day reaching almost 300,000 people a year; Volunteer phytoplankton researchers from 11 different states will be exposed to the ESP at various training workshops; scientifically, the placement of more ESP's into the ocean and eventually onto mobile platforms will give us the potential to inform and drive adaptive sampling in the way that CTD measurements do now; testing and establishing ESP technology will not only further the proposed research but also contribute to commercial interests that manufacture and sell advanced scientific instrumentation. As the technology is proven, other research groups will seek to incorporate it, and demand for the same or similar instruments will grow; many of the enabled/enhanced projects (research and educational) supported by this proposal are multi-investigator, and even multi-institutional, in nature and would further increase networking and partnering among relevant research groups.