EAGER: Rapid Enrichment, Sequencing, and Assembly of Targeted Genomic DNA

EAGER：目标基因组 DNA 的快速富集、测序和组装

• 批准号: 0964859
• 负责人: Michael Axtell
• 金额: $ 10.29万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-15 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0964859&HistoricalAwards=false
• 关键词: EAGER; Rapid; Enrichment; Sequencing; Assembly

Intellectual Merit: Genes are the fundamental unit of biological information. Most genes are discrete segments of DNA which are "expressed" by being used as the templates for RNA production (a process known as transcription); subsequent decoding of the RNA (a process known as translation) results in the production of a protein with a defined function in the cell. Control of gene expression is critical for all organisms; regulation of the initiation of transcription is a critical step at which gene expression is controlled. Regions of DNA flanking transcribed genes often contain short sequences termed cis-regulatory elements (CREs). CREs are bound by proteins which in turn control initiation of transcription from the neighboring gene; this binding typically requires defined DNA sequences within the CREs. CREs which are important maintain a constant sequence over evolutionary time, while the other DNA surrounding them is unconstrained and drifts in sequence. Thus, a powerful method to identify important CREs is to align DNA sequences flanking transcribed genes from multiple, related species. A major technical bottleneck of this approach is determining the flanking DNA sequences themselves. This project seeks to develop a rapid and flexible method to quickly determine the DNA sequence surrounding a gene of interest in multiple species.Broader Impacts: This project involves the training of a female Ph.D. student, and as such, addresses the need to broaden inclusion of women in the Science, Technology, Engineering, and Mathematics (STEM) fields. The methodology itself has the potential to catalyze the discovery of multiple CREs for critical genes. The resulting understanding of gene regulation may in turn have positive impacts in many areas of the biological sciences, as well as potential practical applications in biotechnology.

智力优点：基因是生物信息的基本单位。 大多数基因是DNA的离散段，通过用作RNA产生的模板（称为转录的过程）“表示”。随后的RNA解码（一种称为翻译的过程）导致产生具有细胞中定义功能的蛋白质。 控制基因表达对于所有生物都至关重要。转录开始的调节是控制基因表达的关键步骤。 DNA侧面转录基因的区域通常包含称为顺式调节元件（CRE）的短序列。 CRE被蛋白质结合，蛋白质反过来控制着来自相邻基因的转录启动。这种结合通常需要CRE内定义的DNA序列。 重要的CRE在进化时间内保持恒定序列，而周围的其他DNA则是不受限制的，并且会依次漂移。 因此，识别重要CRE的一种强大方法是将来自多个相关物种转录基因的DNA序列对齐。 这种方法的主要技术瓶颈是确定侧翼DNA序列本身。 该项目旨在开发一种快速，灵活的方法，以快速确定围绕多种物种感兴趣的基因的DNA序列。Boader的影响：该项目涉及对女性博士学位的培训。学生，因此解决了将妇女纳入科学，技术，工程和数学（STEM）领域的必要性。 该方法本身有可能催化为关键基因发现多个CRE的发现。 对基因调节的最终理解反过来可能在生物科学的许多领域以及生物技术中的潜在实际应用中产生积极影响。