MRI: Acquisition of an Illumina HiSeq2000 as a core sequencing instrument for genomics and gene expression research

该主要研究仪器奖支持购买Illumina HiSeq2500 DNA测序仪器，用于宾夕法尼亚州立大学公园校区的基因组学核心设施。DNA测序是现代生命科学研究的重要组成部分。在过去的十年里，DNA测序技术以惊人的速度发展。在此期间，每次仪器“运行”的数据输出增加了1万亿多倍。因此，可用的DNA信息数量激增，使所有生物科学都取得了革命性的进步。Illumina HiSeq2500是迄今为止最强大的测序仪器，无论是单位时间的数据输出，还是每个dna碱基测序的成本。其非常高的质量，相对较长的读取长度，以及完善的测序化学允许最大的实验灵活性。它能够生成DNA序列数据，以允许从头基因组组装，以及重新测序和转录组研究。将这台仪器安装到基因组学设施将大大加强宾夕法尼亚州立大学和其他地方的生命科学研究，特别是在三个广泛的领域：非模式生物的基因组学和基因表达，多物种相互作用的基因组学，以及基因表达的调控。鉴于这一工具的灵活性和强大的性能，这一工具很可能会加强许多其他尚未预见的研究领域。该仪器的功能将极大地加强宾夕法尼亚州立大学的生命科学研究。此外，它还将加强宾夕法尼亚州立大学本科生、研究生和博士后研究人员的跨学科科学培训。它的用途和数据将被整合到现有的实践研究生培训项目和课程中，并作为基因组学核心设施提供的常规系列研讨会的一部分。将通过几个现有的项目，向目前在科学事业中代表性不足的几个学生群体提供机会和培训。在该仪器上进行的实验也将用于加强宾夕法尼亚州立大学继续参与一个以本科院校为主的财团进行科学培训。

This Major Research Instrumentation award supports the purchase of an Illumina HiSeq2500 DNA sequencing instrument for the Genomics Core Facility at Penn State's University Park campus. Sequencing of DNA is a key component of modern life sciences research. In the last decade, technologies for DNA sequencing have advanced at an astonishing rate. In that time frame, data output per instrument "run" has increased by more than a trillion-fold. Consequently, the amount of DNA information available has skyrocketed, enabling revolutionary advances across all of the biosciences. The Illumina HiSeq2500 is the most powerful sequencing instrument available to date, both in terms of data output per unit time, and cost per DNA-base sequenced. Its very high quality, relatively long read lengths, and well-established sequencing chemistry allow the maximum experimental flexibility. It is capable of generating DNA sequence data to allow de novo genome assemblies, as well as re-sequencing and transcriptome studies. Installation of this instrument into the Genomics Facility will significantly enhance life science research at Penn State and beyond, particularly in three broad areas: Genomics and gene expression of non-model organisms, genomics of multispecies interactions, and regulation of gene expression. Given the flexibility and robust performance of this instrument, it is likely that many additional, as yet unforeseen areas of study will be enhanced by this instrument.This instrument's capabilities will dramatically enhance life sciences research at Penn State. In addition, it will enhance interdisciplinary scientific training for Penn State researchers at the undergraduate, graduate, and post-doctoral levels. It's use and data will be integrated into existing hands-on graduate training programs and courses, and used as part of the regular series of workshops offered by the Genomics Core Facility. Access and training will be provided, through several existing programs, to several populations of students who are currently under-represented in the scientific enterprise. Experiments conducted on this instrument will also be used to enhance Penn State's continuing involvement with a consortium of predominantly undergraduate institutions for scientific training.