Development and implementation of advanced methods for the identification of barley flowering time genes using next generation sequencing
使用下一代测序鉴定大麦开花时间基因的先进方法的开发和实施
基本信息
- 批准号:196993113
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Priority Programmes
- 财政年份:2011
- 资助国家:德国
- 起止时间:2010-12-31 至 2014-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Variation in flowering time has always been a major target in breeding programs to adapt crops to a wide range of environments. Deciphering the genetic basis of flowering time variation is thus important for knowledge based breeding of new varieties. Temperate crops like barley and wheat are characterized by large genomes mostly comprising of repeats. Even though these complex genomes are an extreme challenge to all genetic attempts, new technologies allow accessing the gene spaces of these large genomes in a fast and cost-efficient way. Albeit the first reports on simultaneous genetic mapping and mutation identification by next generation sequencing found their way into scientific literature, the continuing bottleneck for high throughput identification of causal mutations remains the appropriate methods and genetic material. Like conventional map-based cloning efforts, such high-speed mapping methods require segregating populations as their primary material. Here we propose to establish two new strategies for mutant identification in barley, which carry the promise to tremendously speed up the generation of mapping populations. First, we propose generation of isogenic mapping populations through self-fertilization of heterozygous mutants as recently proposed for rice. As such mutant plants can be identified within the M2 families of the original screens, it does require one a single generation to establish the segregating population. Second, sequencing of allelic mutants carries the promise to reveal causative genes without any linkage-based analysis, as it is usually only one gene or a very small number of genes that feature severe changes within all allelic mutants. Using nine different allelic series with two to six alleles in each group will unravel the power of this approach. Once established, both of these methods will allow for exploiting the extensive genetic resources of barley mutant populations generated over the last decades.
开花时间的变化一直是育种计划的主要目标,以使作物适应广泛的环境。因此,破译开花时间变异的遗传基础是重要的知识为基础的新品种的育种。温带作物如大麦和小麦的特点是大基因组主要由重复组成。尽管这些复杂的基因组对所有遗传学尝试都是一个极大的挑战,但新技术允许以快速和具有成本效益的方式访问这些大型基因组的基因空间。尽管关于通过下一代测序同时进行遗传作图和突变鉴定的第一份报告已进入科学文献,但高通量鉴定因果突变的持续瓶颈仍然是适当的方法和遗传材料。像传统的基于图谱的克隆工作一样,这种高速作图方法需要分离种群作为其主要材料。在这里,我们建议建立两个新的战略突变体鉴定大麦,进行承诺,大大加快一代作图群体。首先,我们提出了一种通过杂合突变体的自花受精来构建水稻等基因作图群体的方法。由于这些突变体植物可以在原始筛选的M2家族内鉴定,因此确实需要一个单代来建立分离群体。其次,等位基因突变体的测序有望在没有任何基于连锁的分析的情况下揭示致病基因,因为通常只有一个基因或非常少量的基因在所有等位基因突变体中具有严重的变化。使用九个不同的等位基因系列,每组有两到六个等位基因,将揭示这种方法的力量。一旦建立,这两种方法将允许利用在过去几十年中产生的大麦突变群体的广泛的遗传资源。
项目成果
期刊论文数量(0)
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Professorin Dr. Maria von Korff Schmising其他文献
Professorin Dr. Maria von Korff Schmising的其他文献
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{{ truncateString('Professorin Dr. Maria von Korff Schmising', 18)}}的其他基金
Gene identification and functional analysis of high tillering mutants in barley
大麦高分蘖突变体基因鉴定及功能分析
- 批准号:
386675672 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Research Grants
Analysis of genetic variation for flowering time in wild barley grown under different environmental conditions
不同环境条件野生大麦开花时间遗传变异分析
- 批准号:
204994818 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Priority Programmes
The circadian clock and stress adaptation in barley
大麦的生物钟和应激适应
- 批准号:
206837439 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Research Grants
Inflorescence and spikelet meristem establishment and maintenance in barley
大麦花序和小穗分生组织的建立和维持
- 批准号:
468126924 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Units
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