Analysis of genetic variation for flowering time in wild barley grown under different environmental conditions

不同环境条件野生大麦开花时间遗传变异分析

• 批准号: 204994818
• 负责人: Professorin Dr. Maria von Korff Schmising
• 金额: --
• 依托单位: BioScience
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/204994818?language=en
• 关键词: Analysis; genetic; variation; flowering; time

The time of flowering is crucial for a plant’s adaptation to a given environment and has a major impact on grain yield in crop species. A large number of flowering time genes have been identified in A. thaliana, and many of them are structurally conserved across species including cereals. However, the majority of these orthologs have not been functionally characterized in barley or wheat so far. Here, we propose to conduct the first comprehensive survey on variation in flowering time candidate genes and flowering time behavior under different environmental conditions in wild barley, Hordeum spontaneum Thell. We aim at identifying novel associations between candidate genes and flowering time, and to advance the functional characterization of flowering time genes in barley. To this end we will I) characterize genetic variation at 16 candidate genes in a unique collection of 480 diverse barley lines established by the applicant, II) associate genetic variation with flowering behavior under different field and controlled conditions, and III) analyze gene expression and characterize genetic diversity in putative regulatory regions of candidate genes. The characterization of natural genetic variation at flowering time candidate genes will make an important contribution to understanding developmental genetic processes underlying adaptation and thus grain yield in barley and other grasses and increase the pool of alleles available for breeding.

开花时间对于植物适应特定环境至关重要，并对农作物的谷物产量产生重大影响。拟南芥中已鉴定出大量开花时间基因，其中许多基因在结构上在包括谷物在内的物种中是保守的。然而，迄今为止，大多数直系同源物尚未在大麦或小麦中进行功能表征。在此，我们建议对野生大麦Hordeum spontaneum Thell在不同环境条件下开花时间候选基因的变异和开花时间行为进行首次全面调查。我们的目标是确定候选基因与开花时间之间的新关联，并推进大麦开花时间基因的功能表征。为此，我们将 I) 表征申请人建立的 480 个不同大麦品系的独特集合中 16 个候选基因的遗传变异，II) 将遗传变异与不同田地和受控条件下的开花行为联系起来，以及 III) 分析基因表达并表征候选基因的假定调控区域中的遗传多样性。开花期候选基因的自然遗传变异的表征将为理解适应的发育遗传过程以及大麦和其他草类的谷物产量做出重要贡献，并增加可用于育种的等位基因库。