The circadian clock and stress adaptation in barley

大麦的生物钟和应激适应

• 批准号: 206837439
• 负责人: Professorin Dr. Maria von Korff Schmising
• 金额: --
• 依托单位: Department of Biology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/206837439?language=en
• 关键词: circadian; clock; stress; adaptation; barley

The scarcity of water is the greatest problem facing agriculture in the Middle East today. The development of drought resistant crops is a major prerequisite to sustain food production under limited water conditions. Recent studies in Arabidopsis have shown that the circadian clock is an important mechanism controlling stress adaptation in plants by regulating key physiological and metabolic pathways. However, the impact of circadian traits on adaptation and yield potential in a crop has not yet been analyzed. The aims of the proposed project are to elucidate the adaptive significance of variation in the circadian clock in barley and 2) to gain a mechanistic understanding of the barley circadian clock and its interrelationship with the metabolome and abiotic stress. In the first phase of the project, we have successfully established a high-throughput system to measure circadian rhythmicity based on changes in fluorescence (F) and identified clock mutants in the wild barley collection B1K. In the second phase of the project we propose to use the identified clock variants to a) define output traits of the clock such as physiological and agronomic performance (yield) in the field and under controlled conditions and b) to define input pathways into the clock by testing plasticity of the clock in response to abiotic stresses. In addition, we propose to analyse circadian changes in global gene expression, carbon metabolism and physiology to reveal how the clock regulates responses to environmental changes in barley. Variation in global gene expression, carbon metabolism and physiology will be integrated in a comprehensive network to identify causal relationships, input signals and output parameters of the clock and its effects on performance. Novel genes/alleles and germplasm resources can be used for marker assisted selection of barley adapted to dry environments in the Middle East and will be also valuable for precision breeding of barley adapted to climate change in temperate latitudes.

水资源短缺是当今中东农业面临的最大问题。发展抗旱作物是在有限的水资源条件下维持粮食生产的一个主要先决条件。近年来对拟南芥的研究表明，生物钟是植物逆境适应的重要机制，它通过调节重要的生理代谢途径来实现。然而，尚未分析昼夜节律性状对作物适应性和产量潜力的影响。 该项目的目的是阐明大麦生物钟变化的适应意义，2）获得大麦生物钟及其与代谢组学和非生物胁迫的相互关系的机制理解。在该项目的第一阶段，我们成功地建立了一个高通量系统，以测量基于荧光（F）的变化的昼夜节律，并确定了野生大麦B1 K中的时钟突变体。在该项目的第二阶段，我们建议使用已识别的时钟变体来a）定义时钟的输出特征，例如田间和受控条件下的生理和农艺性能（产量），以及B）定义输入途径通过测试时钟响应非生物胁迫的可塑性来定义时钟。此外，我们建议分析全球基因表达，碳代谢和生理的昼夜变化，以揭示生物钟如何调节大麦对环境变化的反应。全球基因表达，碳代谢和生理学的变化将被整合到一个综合网络中，以确定因果关系，时钟的输入信号和输出参数及其对性能的影响。新的基因/等位基因和种质资源可用于标记辅助选择大麦适应干旱环境在中东地区，也将是有价值的精确育种大麦适应气候变化在温带纬度。

项目成果

The Impact of Domestication on the Circadian Clock.

驯化对昼夜节律时钟的影响

• DOI: 10.1016/j.tplants.2016.01.022
• 发表时间: 2016
• 期刊: Trends in plant science
• 影响因子: 20.5
• 作者: Shor E;Green RM
• 通讯作者: Green RM

Mutation at the circadian clock gene EARLY MATURITY 8 adapts domesticated barley (Hordeum vulgare) to short growing seasons

• DOI: 10.1073/pnas.1120496109
• 发表时间: 2012-05-22
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Faure, Sebastien;Turner, Adrian S.;Laurie, David A.
• 通讯作者: Laurie, David A.