Patterning Interfaces with High Molecular Weight Periodically Sequenced Sheet-Forming Polypeptides

与高分子量周期性排序片状形成多肽的图案化界面

• 批准号: 1006407
• 负责人: Raymond Tu
• 金额: $ 32.46万
• 依托单位: CUNY City College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2015-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1006407&HistoricalAwards=false
• 关键词: Patterning; Interfaces; Molecular; Weight; Periodically

ID: MPS/DMR/BMAT(7623) 1006407 PI: Tu, Raymond ORG: CUNY City CollegeTitle: Patterning Interfaces with High Molecular Weight Periodically Sequenced Sheet-Forming PolypeptidesINTELLECTUAL MERIT: The goal of the proposed work is to create a periodically sequenced polypeptide with a high molecular weight and a low polydispersity for patterning self-organized nanostructures confined at interfaces. Previous efforts in the PI's group to template interfaces with low molecular weight periodically sequenced peptides have yielded surface confined self-organized fibrillar patterns, but these patterns have a low aspect ratio and a high defect frequency. It is proposed here to use a condensation reaction to promote a high molecular weight periodically sequenced polypeptide (PSP). Typically, the polydispersity of the condensation polymerization process is determined by the statistics of recombination, leading to a broad distribution. The approach proposed for making these polypeptides with a high molecular weight and a narrow polydispersity is based on controlling the condensation kinetics of a growing beta-strand in a multiphase transport-limited reaction. The mechanism to synthesize a pseudo-living condensation polymer with narrow distribution is an outcome of three key phenomena: cooperative folding, Langmuir adsorption kinetics, and effective reaction rates that decrease as a function of molecular weight. To characterize the reaction products the PI will apply a combination of composition gradient-multi-angle light scattering, NMR, and LC-MS. To characterize assembly at the molecular length scale, Langmuir-Blodgett techniques, circular dichroism, and attenuated total reflectance-FTIR will be applied. Brewster angle microscopy, imaging ellipsometry, and fluorescent microscopy will be used to characterize assembly at the supramolecular length scale.BROADER IMPACTS: The proposal may lead to new, less expensive methodology for producing polypeptides of controlled sequence and chain length. It will support the work two graduate students and several undergraduate students. In fact, two undergraduate students conducted all the preliminary experiments upon which the proposal is built. Undergraduates will continue to participate through three distinct mechanisms. First, the PI participates in two NSF sponsored undergraduate education programs (1) NSF-NUE (Nanomaterials Education for Engineering and Science Majors at CCNY) (NSF EEC 0634223) and (2) NYU-CCNY, REU for the Science and Engineering of Soft Materials and Interfaces (NSF DMR 0648788). The NUE is designed to enhance nanotechnology and nanomaterials education for undergraduate students at CCNY. The REU is in collaboration with NYU, and the interdisciplinary focus is soft materials and interfaces. These outreach programs will expose students, who typically don't have opportunities in science and engineering, to academic research. Second, the PI aspires to provide quality undergraduate training to a highly diverse student body at CCNY (36% Hispanic, 30% black, 23% Asian and 11% white) through direct participation in his laboratory. Among the twenty students who have worked in the PI's lab eleven are women, including two Hispanic women and two African American women. The PI is dedicated to the development of a diverse laboratory environment, which is in accord with the CCNY mission. Third, the PI is integrating research and education in a comprehensive fashion from the graduate to the high school level. To this end, the PI is leading a Peer-Learning Program with the High School of Math, Science and Engineering (HSMSE) in Harlem, NY. The program has two goals: first, to excite and recruit students from a diverse community into STEM fields and, second, to engage more engineering students in teaching. The program propagates knowledge by giving undergraduate students at CCNY an opportunity to teach chemical engineering at HSMSE. Peer interactions foster a positive learning environment where interest in science and engineering is promoted through shared everyday experience. This notion of common ground is particularly important because the diverse population at HSMSE currently mirrors the makeup at CCNY.

ID：MPS/DMR/BMAT(7623) 1006407 PI：Tu，Raymond 组织：纽约市立大学 标题：与高分子量周期性片状形成多肽的图案化界面 知识优点：拟议工作的目标是创建一种具有高分子量和低多分散性的周期性排序多肽，用于图案化自组织纳米结构 限制在界面处。 PI 小组之前用低分子量周期性测序肽模板界面的努力已经产生了表面限制的自组织纤维图案，但这些图案具有低纵横比和高缺陷频率。这里建议使用缩合反应来促进高分子量周期性测序多肽（PSP）。通常，缩聚过程的多分散性由重组的统计数据决定，导致广泛的分布。所提出的用于制备这些具有高分子量和窄多分散性的多肽的方法是基于控制多相传输限制反应中生长的β链的缩合动力学。合成具有窄分布的拟活性缩聚物的机制是三个关键现象的结果：协同折叠、朗缪尔吸附动力学以及随分子量而降低的有效反应速率。为了表征反应产物，PI 将结合使用成分梯度多角度光散射、NMR 和 LC-MS。为了在分子长度尺度上表征组装，将应用 Langmuir-Blodgett 技术、圆二色性和衰减全反射 FTIR。布鲁斯特角显微镜、成像椭圆光度术和荧光显微镜将用于表征超分子长度尺度的组装。 更广泛的影响：该提案可能会带来新的、更便宜的方法来生产受控序列和链长的多肽。 它将支持两名研究生和几名本科生的工作。 事实上，两名本科生进行了该提案所依据的所有初步实验。 本科生将继续通过三种不同的机制参与。 首先，PI 参加两个 NSF 资助的本科教育项目 (1) NSF-NUE（CCNY 工程和科学专业纳米材料教育）(NSF EEC 0634223) 和 (2) NYU-CCNY、REU 软材料和界面科学与工程 (NSF DMR 0648788)。 NUE 旨在加强 CCNY 本科生的纳米技术和纳米材料教育。 REU 与纽约大学合作，跨学科重点是软材料和界面。 这些外展计划将使通常没有科学和工程机会的学生接触到学术研究。 其次，PI 渴望通过直接参与他的实验室，为 CCNY 高度多元化的学生群体（36% 西班牙裔、30% 黑人、23% 亚洲人和 11% 白人）提供优质的本科生培训。 在 PI 实验室工作的 20 名学生中，有 11 名是女性，其中包括两名西班牙裔女性和两名非裔美国女性。 PI 致力于开发多样化的实验室环境，这符合 CCNY 的使命。 第三，PI正在从研究生到高中阶段全面整合研究和教育。 为此，PI 正在与纽约州哈莱姆区的数学、科学与工程高中 (HSMSE) 一起领导一项同伴学习计划。 该计划有两个目标：首先，激发和招募来自不同社区的学生进入 STEM 领域；其次，让更多的工程专业学生参与教学。 该计划通过为 CCNY 的本科生提供在 HSMSE 教授化学工程的机会来传播知识。 同伴互动营造了积极的学习环境，通过分享日常经验提高对科学和工程的兴趣。 这种共同点的概念尤其重要，因为 HSMSE 的多元化人口目前反映了 CCNY 的构成。