EAGER: Collaborative Research: Mimicking mussel adhesion with periodically sequenced polypeptides

EAGER：合作研究：用周期性测序的多肽模拟贻贝粘附

• 批准号: 1506539
• 负责人: Raymond Tu
• 金额: $ 5.95万
• 依托单位: CUNY City College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1506539&HistoricalAwards=false
• 关键词: EAGER; Collaborative; Research; Mimicking; mussel

1506539 (Tu) / 1508717 (Waite)The proposed work emphasizes discoveries in bioadhesion, which has the potential to have "far reaching effects" in a number of technologies. Moreover, the ability to generate a significant quantity of a non-toxic biomimetic adhesive has excellent potential for STEM education, where simple demonstrations can be developed to engage students in fundamental science questions as well as the engineering of materials. Additionally, CCNY is a Minority Serving Institution, and the proposed interdisciplinary work is ideal for engaging students at the high-school, undergraduate and graduate levels. The PI has developed a "community" of science teachers at Harlem area schools, where area teachers can work together with high school students in the research lab during the summer and school year. Taken together, the proposed work can both broaden participation in the local STEM community as well as lead to a significant impact in the adhesion and interfacial science community.Marine mussels use the catecholic amino acid 3,4-dihydroxyphenylalanine (Dopa) in mussel foot proteins to mediate robust wet adhesion in the turbulent intertidal zone. The objective of this EAGER proposal is to quantitatively explore the role of high fidelity sequence periodicity in Dopa-rich polypeptide on the dynamics of coating and adhesion. Moreover, the PIs propose a nonbiological synthetic protocol for the synthesis of a high molecular weight periodic sequence with a low polydispersity, allowing us to synthesize bulk-scale inexpensive polymer. Our approach is based on the application of a transport-limited polycondensation reaction to define both the periodicity and the polydispersity. Subsequently, the PIs propose to quantify the coating dynamics and work of adhesion as a function of the sequence parameters. They will use developed synthetic protocols to precisely control the role of Dopa/proline frequency and the spatial distribution of peptides. This research team has established expertise in both the synthetic methods and the characterization tools, but the proposed work aims at EAGER funding because the vision of marrying polymer-scale synthetic tools and biomimetic adhesion is untested and involves a radically different approach.

1506539(图)/1508717(WEITE)拟议的工作强调了生物附着方面的发现，这可能会对许多技术产生“深远的影响”。此外，产生大量无毒仿生粘合剂的能力对STEM教育具有极大的潜力，可以开发简单的演示来吸引学生回答基础科学问题和材料工程。此外，CCNY是一个少数民族服务机构，拟议的跨学科工作是吸引高中、本科生和研究生的理想选择。PI在哈莱姆地区的学校建立了一个科学教师社区，在那里，该地区的教师可以在暑假和学年期间与高中生在研究实验室一起工作。综上所述，这项拟议的工作既可以扩大当地STEM社区的参与，也可以在粘附界和界面科学界产生重大影响。海洋贻贝利用贻贝足部蛋白质中的儿茶酚氨基酸3，4-二羟基苯丙氨酸(DOPA)在湍急的潮间带调节强大的湿粘着。这个急切的提议的目的是定量地探索富含多巴的多肽中的高保真序列周期性对涂层和黏附动力学的作用。此外，PI提出了一种非生物合成方案，用于合成具有低多分散性的高分子量周期序列，使我们能够合成本体规模的廉价聚合物。我们的方法是基于输运受限的缩聚反应来定义周期性和多分散性。随后，PI建议将涂层动力学和附着力功作为序列参数的函数来量化。他们将使用开发的合成方案来精确控制多巴/脯氨酸频率的作用和多肽的空间分布。这个研究团队在合成方法和表征工具方面都建立了专业知识，但拟议的工作旨在获得急切的资金，因为将聚合物规模的合成工具和仿生粘合结合起来的愿景未经测试，涉及一种截然不同的方法。